Mokhoa oa ho sebetsana le litaba tse tloaelehileng tse tloaelehileng tse nang le li-vaiaso tsa kelello tsa 2ml autosampler

Li-vielis tse Viamectke likarolo tse nyarosang tsa k'hemistri tsa tlhaiso-leseling, haholo-holo ka har'a li-chromatograph tsa chromatography tse ngata (HPTC) le li-chromatograph tsa khase (gc). Liphaephe tsa 2ml a boitsebiso tsa 2ml li e 'ngoe ea boholo bo sebelisoang haholo, empa litabeng tse fapaneng li ka hlaha nakong ea ha ba sebelisoa. Tataiso ena e fana ka kakaretso e felletseng ea mathata a tloaelehileng a amanang le li-vaianos tsa 2ml autosampler li fana ka tharollo ea mathata ho netefatsa ts'ebetso e nepahetseng.

Sebopeho sa li-vial tsa li-apole tsa 2ml autosampler

Pele u kenella ho rarolla bothata, ho bohlokoa ho utloisisa sebopeho sa mantlha le tšebetso ea li-vaesamp tsa la 2ml lipolocoler. Li-vielis tsena li akaretsa:

Boitsebiso: Boholo bo entsoe ka khalase e boromisicate, e loantšanang ka lik'hemik'hale ebile e fokotsa tšebelisano ka sampole.

Mofuta oa Cap: Likhetho li kenyelletsa li-screw Caps, li-cap tsa crimp, le li-caps tse ngata, e mong le e mong ea nyehelang mekhoa e fapaneng ea ho tiisa mekhoa e fapaneng.

Septa: karolo e nyarosang e lumellang Polonteraka ea nale ha o ntse o boloka tiiso ea ho thibela ho ba mouoane kapa litšila.

Wan Ho Tseba Tsebo e Feletsoeng mabapi le Tsela ea ho Hloekisa Liphatlalatso tsa Chromatography, ka kopo sheba sengoloa sena: Ka ho sebetsa hantle! Mekhoa e 5 ea ho hloekisa li-chromatography tsa mohlala oa mohlala

Mathata a Tloaelehileng le Mehato e Tharollang

1. Ho feta tekano

Bothata: Ho feta tekano ho etsahala ha palo ea mokelikeli ka temo e feta. Sena se baka sebaka se sa lekaneng ea lihlooho bakeng sa nale ea Autosampler hore e sebetse hantle.

Matšoao: ente e sa khoneheng ea mohlala, ise ea satisever, e eketsehang ea likepe tse eketsehang kapa nale e senyehileng.

Tharollo: Etsa bonnete ba hore vial e tlala boemo bo khothalelitsoeng, hangata e ka ba 1.5ml bakeng sa2ml kal. Sena se fana ka lihlooho tse lekaneng bakeng sa ho kenella ha batho ba bangle le samle.

2. TLHOKOMELISO EA mohlala

Bothata: Likalimo li ka hlaha ho tsamaisa ka mokhoa o sa lokang, ho tiisa matso a ka tlase kapa ho tiisoa.

Matšoao: litlhoro tse sa lebelloang ho chromatograph kapa sampole ea samromatogram kapa e fetotsoeng.

Tharollo: Ts'ebelisoLi-vial tsa boleng bo holimo le li-capetselitsoe kopo ea hau e khethehileng. Kamehla sebetsana le li-gloves le liatlana 'me u qobe ho ama libaka tse ka hare. Nahana ka ho sebelisa liphaephe tse netefalitsoeng esale pele kapa tse netefalitsoeng ho fokotsa kotsi ea tšilafalo.

3. Lithaba tse sa lebelloang ho chromatograph

Bothata: Litlhōrō tse sa lebelloang li ka supa tšilafalo kapa litsamaiso tsa lisepo tse fetileng.

Mehato ea mathata:

Metsoako e hloekileng ea aufosampler khafetsa: Ho kenyelletsa kemiso ea ho hloekisa letsatsi le letsatsi ea likarolo tsa autosampler, ho kenyelletsa li-syringes le li-syringes.

Sebelisa likhakanyo tse tlase tsa molumo: Khetha li-vaell tsa tsoelo-pele tse phahameng tse etselitsoeng ho fokotsa bophahamo ba molumo ebe kahoo fokotsa kotsi ea Crossover.

4. Blakage ea Nale

Bothata: blockAge e ka hlaha ka lebaka la likarolo kapa mohlala oa Vincous o tla thibela nale ea Autosampler.

Matšoao: Ha ho sampole e kentsoeng kapa bophahamo ba molumo o thellisa.

Tharollo: Fillight Mohlala o sebelisa pampiri e loketseng kapa membrane ho tlosa likarolo tse ngata tse mpe. Haeba blackge e etsahala, hloekisa nale ho latela litaelo tsa moetsi 'me u nahane ka nale e kholo ea maikutlo a batho ba nkang maikutlo.

5. Ho arola Via

Bothata:Khalase kalsE ka 'na ea phomola haeba e ikuta khatellong e feteletseng, e ts'oeroang, kapa ho ts'oaroa hampe.

Matšoao: Tšenyo ea 'mele e bonahala ka tlhahlobo ea tlhahlobo; Mohlala o kanna oa lahleha.

Tharollo: Tšoaea ka hloko le ho qoba liphetoho tsa mocheso. Sebelisa sekoaelo sa ts'ireletso haeba ho hlokahala nakong ea lipalangoang kapa polokelo.

6. Ho tšepahala hoa tiiso

Bothata: Litiiso tse sa lekaneng li ka lebisa ho mouoane oa likarolo tse sa fetoheng kapa litšila tse tsoang mehloling ea kantle.

Matšoao: Liphetoho ho sekaseka mahlorisong a fetileng; tahlehelo ea metsoako e sa fetoheng.

Tharollo: Ho netefatsa hore libapali li tsamaisana le mofuta o boima ebile li tiisitsoe hantle. Haeba ho sebetsana le mehlala e makatsang, nahana ka ho sebelisa li-cap tsa crimp bakeng sa botšepehi bo ananelang.

U batla ho tseba ho khetha cap e nepahetseng bakeng sa valiagraphy ea hau ea chromatography, ka kopo sheba sengoloa sena: U ka khetha cap e nepahetseng joang bakeng sa likhama tsa hau tsa chromagraphy?

7. Ho se lumellane le Autosamplemps

Bothata: Ha se li-vaels tsohle li tsamaisana le mohlala o mong le o mong oa ho reka o ka bakang litaba tsa mochini nakong ea ts'ebetso.

Matšoao: Ho sebelisa boitšoaro bo bobe kapa ho sitoa ho kenya disampole.

Tharollo: Netefatsa ho lumellana ha mofuta o boima le mohlala oa autosampler pele o sebelisoa. Sheba tataiso ea moetsi bakeng sa mashano a khothalletsoang.

8. Auturgiation ea mocheso

Bothata: Liphetoho tsa mocheso nakong ea polokelo kapa tlhahlobong e ka ama botsitso ba mohlala, haholo-holo bakeng sa metsoako ea mocheso.

Matšoao: Ho phatloha ha sekaseka ho lebisang liphethong tse sa lokang.

Tharollo: Boloka mehlala ea mocheso o khothalelitsoeng le ho fokotsa litlamorao tsa mocheso nakong ea ho hlahlojoa.

Ho khetha li-vavers tse nepahetseng tsa 2ml AutoSosopler

Ho fokotsa litaba le likhama tsa 2ml a sa na thuso, nahana ka ho kenya mekhoa e latelang e ntle:

Tlhokomelo ea kamehla le calibrance: Runisa ho lokisa khafetsa ho AutoSompler ea hau le ho etsa likatse ho latela tataiso ea moetsi.

Maemo a nepahetseng a polokelo: Boloka li-vaiss tikolohong e hloekileng, e omileng hole le khanya ea letsatsi le lithempa tsa lithemparetjha tse feteletseng.

Ho khetha mofuta o nepahetseng oa lekhetho bakeng sa kopo ea hau: ho latela kopo ea hau (HPTC vs.PO E MOLEMO


Batla ho tseba likarabo tse 50 mabapi le li-valc vials, ka kopo sheba sengoloa sena:Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

Ho bua ka litaba tse tloaelehileng le2ml autosamplerHo bohlokoa ho boloka ho nepahala le ho ts'epahala ka liphetho tsa hau tsa sekaseka. Ka ho utloisisa litaba tse kang tse ling tse kang ho tepella ka bongata, ho qoelisoa ka mokhoa o sa tšoaneleng, li-book. Ho kenya mekhoa e metle ka ho sebetsana le ho sebetsana le eona, ho boloka le ho lokisa le ho lokisa ho tla ntlafatsa ts'ebetso ea tsamaiso ea hau ea ho u amosahale. Koetliso ea kamehla ea basebetsi ba laboratoring e libakeng tsena e ka fokotsa liphoso tse amanang le tšebeliso ea bohlanya ea chelete e fokolang.