Melema e bonolo ea ho sebelisa li-chromatography: Malebela a 10 bakeng sa ts'ebetso ea boreleli
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Melema e bonolo ea ho sebelisa li-chromatography: Malebela a 10 bakeng sa ts'ebetso ea boreleli

Sep. 6th, 2023
Mokhoa oa chrotography ke mokhoa oa bohlokoa oa laboratoring o sebelisitsoeng ho arohana le ho se fumane lithethefatsi le tlhahlobo ea tikoloho le tlhahiso ea lijo. Bakeng sa liteko tse ntlafatsang tsa chromagraphic, litsomi tsa boleng bo holimo li lokela ho sebelisoa; Sehloohong sena re tla arolelana likeletso tsa ho li sebelisa hantle.

Bohlokoa ba liphapang tsa chromatography

Lithako tsa ChromatograftNa lisebelisoa tse entsoeng ka mokhoa o itseng li sebelisetsoa limokelo tsa chromatographic tse kang chromatographs tsa khase (GC), bakeng sa li-chromographs tse ling tsa chromographic. Boleng ba bona bo ka ba le phello e kholo mabapi le sephetho sa liteko; Ke Hobane'ng ha e le taba e ngata:

Botšepehi ba Mohlala:Litomana tsa li-chromatography li lokela ho boloka botšepehi ba disampole ka ho thibela tšilafalo kapa mouoane, e ka fetolang litaba tsa eona le ho inehela ho sekaseka ho nepahala ha tsona. Phetoho efe kapa efe e ka etsa ho nepahala.

Ho lumellana:Li-vaels li lokela ho lumellana le sistimi ka bobeli le sampole ea li-slivent tsa chrotographic ho hlakisoa ntle le ho phatloha kapa mathata a mang a sebetsang. Liphate tse sa lumellaneng li ka fella ka ho tlosoa kapa mathata a mang a sebetsang a sitisang katleho.

Ho hlahisa tlhahiso:Boleng bo boholo bo ka ba le phello e kholo ho hlahisa sephetho sa litholoana, 'me ka ho khetha likhakanyo tse ngata tsa liteko tse ka ithutoang ka tieo.

Malebela a 10 bakeng sa ts'ebetso e boreleli


Kaha joale re utloisisa bohlokoa ba litakatso tsa camatography, a re ke re tšohleng likeletso tse ling tsa ts'ebetso ea lab e boreleli:

Khetha thepa e sa sebetseng hantle:Malinyane a chromography a tloaelo a entsoe ka khalase kapa polasetiki; Khalase e be e le ka hare mme o loketse ho sebelisa haholo ha polasetiki e ne e khethoa bakeng sa lihlahlobo tse ikhethang. Etsa bonnete ba hore mokhoa oa hau oa sampole le tlhahlobo o ka amohela mofuta o mong oa mofuta o mong le o mong oa mapolane.

Na u thahasella ho utloisisa melemo ea likhahla tsa chromatography tsa chromatography bakeng sa li-varomatographs tsa chromatography lintja tsa polasetiki? Sheba sehlooho sena:Mabaka a 3 a holimo ho 3
Khetha cap e loketseng: Li-cap tsa vaeraTloo ka har'a lisebelisoa tse fapaneng tse kang aluminium, polasetiki le rabara; Ho khetha thepa e nepahetseng ka ho fetisisa ho ka ba le tšusumetso e tšusumetso ho latela botšepehi le ho e latela. Li-caps tse fetileng kapa li-slap tse phahameng li fana ka tharollo e bonolo 'me kamehla li lokela ho khethoa ha li ntse li fana ka linako tse sireletsehileng tsa linako tse sireletsehileng.

U batla tataiso mabapi le hore na u khetha motho ea thetsang pele kapa che? Ka kopo sheba sengoloa sena:U ka khetha septa pele ho joang kapa che?
Kgetha Li-vaialified tse netefalitsoeng:Liphaero tsa boleng bo netefalitsoeng li na le taolo ea boleng bo tiileng pele u ka sebelisa, ho thusa ho boloka nako le ho fokotsa likotsi tsa liphoso tsa liteko. Ka ho e boloka li-vaerase tse netefalitsoeng tseo u ka li bolokang lijaki hammoho le monyetla oa liphoso tse etsahalang nakong ea liteko.

Malinyane a hloekileng:Kamehla netefatsa hore li-vae li se li hloekisitsoe hantle pele li sebelisoa ho tlosa metanol, tse kang methanito kapa acetone, pele a li hlatsoa kotsing efe kapa efe ea ho silafatsa tšilafalo.

E na le thahasello ea ho fumana leseli le akaretsang la ho hloekisa li-cutiling tsa chromatography tsa ho hloekisa ciaromanography? Se ke oa hloloheloa sengoloa sena se molemo:Ka ho sebetsa hantle! Mekhoa e 5 ea ho hloekisa li-chromatography tsa mohlala oa mohlala

Ho sebetsana ka setotsoana ho Vias:Ho ba hlokolosi e eketsehileng ha u sebetsana le likhathatso tsa carmatography ho qoba ho khesoa kapa ho senyeha, ho sebelisa li-racks tsa likotsi tse ka fokotsang monyetla oa likotsi tse etsahalang.

Label lierls hantle:Label each vial clearly with essential information, such as sample name, concentration level and date, to ensure traceability and prevent mix-ups. Ka ho etsa sena, ho ngola ho ka khona ho thusa ho thula ha le ho thusa ho thibela ho ferekana litho tsa sehlopha sa Paki.

Lekola ho tsoa: Pele u kenya sampole ea hau, cheke ea pele bakeng sa ho tsoa ka tlhōlo e haufi ea ho tsoa. Tiisa li-cap ka mokhoa o sireletsehileng ha o ntse o le hlokolosi a se ke a tiisa ka hona ka ho senyeha kapa hona ho baka ho tsoa ho li-vials.

Boloka litemana ka nepo:Li-vaels li lokela ho bolokoa tikolohong e se nang khanya ea letsatsi le lithemparetjha tse feteletseng e le hore li tla phela bophelo ba tsona ba sehatsetsi, 'me tsa boloka botšepehi ba bona. Ho boloka hantle ho eketsa bophelo ba sehakhoe le ho boloka botšepehi ba mohlala.

Lahla li-vielis tse sireletsehileng:Latela melaoana e nepahetseng ea polokeho ea laboratori le melaoana ea tikoloho ha o la lahla likhama tse sebelisoang, ho kenyelletsa ho nchafatsa likhetho tseo ho ka khonehang. Ho bohlokoa hore lits'ebetso tse nepahetseng li bonoe.

Tebello ea Tefo ea Sepaka Kamehla:Le ka falimehe mabapi le ho hlahloba liphallelo tsa hau tse tebileng, 'me sebaka li tlaleha pele ho liteko tsa liteko.

Ho latela litaelo tsena ho ka netefatsa hore oa hauLithako tsa ChromatograftTlatsetsa katleho le ho ts'epahala ha liteko tsa hau. Le lintlha tse nyane, joalo ka ho khetha boitsebiso kapa ho sebelisa hantle ho ka ba le phello e kholo ho hore na li-chromatograkgraphic ha li fetohe.

Sephetho


Lithibelo tsa li-chromatography ke likarolo tsa bohlokoa tsa mekhoa ea sejoale-joale ea li-chromatograph, le khetho ea bona le ho sebetsana le tsona e bohlokoa bakeng sa ts'ebetso ea mesebetsi e boreleli ea laboratoring. Ka ho ela hloko ka ho hlaka boleng ba boleng bo boima, bohloeki le mekhoa ea polokelo eo u ka ntlafatsang ho nepahala le liteko tsa liteko tse bakiloeng ka lebaka la litebello tse tšepahalang le tse nang le morero.

Lekola tataiso e phethahetseng ho ea holimo ho lipotso tse holimo tse 50 tse botsoang khafetsa ka li-pilc vaiations tsa HPLC 'me u fumane litaba tsa bohlokoa:Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang
E fetileng:
Melemo ea 8 ea Bohlokoa ea Liphate tsa Chemromatography tse tlase
E 'ngoe:
Mokhoa oa ho tlatsa ka nepo likhama tsa chromatography bakeng sa liphetho tse 10
Litags: li-hipsamp-appsopler-vials Li-hplc-vials-vials-vials-vials hipls-vials hplc-valia li-khalase tsa likhalase hplc-2ml-amber-valias Vias-chromatography-2l Likokoana-hloko tsa khalase li na le li-screw-cap screw-vial
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