U ka khetha joang pakeng tsa hpl le GC? Ka papiso e tebileng ea lisebelisoa le tumellano

Kenyelletso

Lithuto tsa chrotographic, ho khetha sampole e nepahetseng ho fana ka tšusumetso e nepahetseng le ho fana ka bonnete ba liphetho tsa hau. Sengoliloeng sena se fana ka phello e felletseng ea liphapang tse ngata tsa hpc le tsa GC

Ke fapanyetsana liphapang pakeng tsa hipc le li-gc tse litšila

1. Tsitso ea Sesebelisoa

Li-pilc tsa hipc: Haholo-holo e sebelisoa ho li-chromatograph tse phahameng tsa chromatography (HPTC) le li-hang-hang tsa LC-MS, tse loketseng bakeng sa mehlala e sa tloaelehang, e sa tsitsang e sa tsitsang.

Gc vaials: E sebelisoa ka li-chromatography tsa khase chromatography

2. Litaba le merero ea meralo

Mefuta ea Motlani ea Val:

HPTC:Hangata sebelisa li-tops tse khopisang kapa li-tops tse phahameng, tse loketseng ho fihlella khafetsa le sampole.

GC:Hangata e bolotsana e kholo ea li-cals tse phahameng ka li-cap tsa aluminium li le li-cap tsa aluminium

U batla ho ithuta ka mekhoa e 5 ea ho hloekisa li-cumanography tsa mohlala? Tobetsa mona bakeng sa tataiso e qaqileng

Khetho ea Boitsebiso:

HPTC:Ka tloaelo e entsoe ka khalase e matla ea bohloeki e matla, ka mefuta ea linaha tsa naha e bonolo bakeng sa mehlala e metle.
GC:E hloka khalase e hanyetsanang e hanyetsanang e tloaelehileng e tloaelehileng, e nang le polasetiki ea pfa e sebelisoang lits'ebetsong tse ikhethang.

II. Lintlha tsa Bohlokoa tsa Malinyane a Viamatography

1. Lisebelisoa tsa khalase

Khalase ea borolicate: Ente e metle le mocheso mocheso; e loketse tlhahlobo ea li-hplc le GC.
Khalase ea Amber: E fana ka tšireletso e bobebe bakeng sa sampole e bobebe e bobebe.

2. Lisebelisoa tsa polasetiki

Polyproplene (PP): E theko e boima ebile e loketse tšebeliso ea mocheso o tlase; ho tsitsisa ha ho na le moeli le khatello ea mocheso.
Perfluoroalkoxy (PFA): Ho hanyetsa ha ho hanyetsa mekhoa e metle ea tlhaho; E loketseng mokhoa oa ho tela le ho fumana tlhahlobo e phahameng ea GC.

Batla ho ithutaKe eng ea GC e shebaneng? Tobetsa mona bakeng sa ho shebahala ka botebo

3. Lisebelisoa tsa cappa le Septa

PTF \ Sillicone Septa: Thibela ho kenella ha Solao ho Hplc; Boloka sebopeho tlasa mocheso ho GC.
Mekhoa e Hlahang: Li-Crimp tsa Crimp li hloka lisebelisoa tse khethehileng, ha ho bonolo hore li-caps tsa HPLC li bonolo ho li sebetsana.

III. Litaba tse mabapi le ho li qoba

1. Lisebelisoa tse fetohang

Khokahano ea AutoSoTor: Netefatsa bophahamo ba Vialista le bophara ba mofuta oa "
Septa Pubture Han: Bakeng sa liente tsa khafetsa ho HPTC, khetha Septa ka Elaysticity (e.g., PTFE \ silicone copestete).

Ho tobana le li-cap tsa vaera le Septa, o hloka ho tseba Pleak mona

2. Taolo e kotsing ea kotsi

Ho qoba tse ikhethang: Bakeng sa mocheso o phahameng oa GC, khetha khalase ea khalase kapa piala.
Ho thibela tšilafalo ea Cross-CrossSebelisa li-vaialisi tse netefalitsoeng pele li hloekisitsoeng pele (e.g., sehlopha sa USP A).

IV. Pretading ea ho reka: Ho khetha ho letsa le letona bakeng sa HPLC le GC

Hlalosa litlhoko tsa liteko: Nahana ka mocheso oa mocheso, mohlala oa mohlala oa sampole
Kopanya lintlha tsa lisebelisoa: Netefatsa mofuta o nepahetseng oa molala, molumo, le ho tsamaisana le ho lekana.

Ha ho joaloCrimp Via Val Vs Snap vs screw cal vaal, Tsela ea ho Khetha? Master Motsamaisi oa Khetho ea Khetho ea Khetho

Sheba maemo a indasteri: Sebelisa li-vielis tse nang le Setifikeiti sa US kapa sa ISO bakeng sa ho feto-fetoha ha boleng.
Tlhahlobo ea Rual-Rualth: Bakeng sa liteko tsa khafetsa, khetha likhahla tse theko e tlaase tsa khalase; Bakeng sa tlhahlobo ea ho ts'oara, khetha bakeng sa lisebelisoa tsa kahare.

V. Faq - Lipotso tse tloaelehileng li arajoa

Q: Malinyane a Hplc a ka sebelisoa bakeng sa tlhahlobo ea GC?
K: Haeba e entsoe ka khalase ea borolilicate le mocheso mocheso (e.g., ho fihlela ho 400 ° C), li ka sebelisoa ka nakoana. Leha ho le joalo, tšebeliso ea nako e telele tlasa mocheso o phahameng ha o khothalletsoe.

Q: Joang ho qoba ho hlekefetsoa ka tsela e telele?
A: Khetha li-vials tse netefalitsoeng esale pele kapa li li hloekisa u sebelisa li-solvet tsa chromatographic-grade.

Q: Na li-vimp tse phahameng li hlokahala bakeng sa GC?
A: Li-vaels tsa Crimp li fana ka matla a phahameng haholo mochesong o phahameng, leha lisebelisoa tse ling li tšehetsa likhoele tsa screw ka bafani.

Batla ho tsebaMokhoa oa ho hloekisa HeadPamon Via? Tobetsa mona ho hlahloba hape

Sephetho

Ho khetha li-hplc tse nepahetseng tsa HPPT kapa GC Ka ho ntlafatsa lisebelisoa le litlhaloso tsa vae, o ka ntlafatsa haholo data ea data le ho fokotsa litšenyehelo tsa liteko tsa liteko.