Ts'ebetso e phahameng ea ts'ebetso ea li-chromatography (HPLC) le ion chromatography

Batla ho tseba likarabo tse 50 mabapi le li-valc vials, ka kopo sheba sengoloa sena:Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

Mechanism ea karohano

HPTC e sebelisana le lits'ila tsa hydrophobic kapa tsa hydrophilic bakeng sa karohano. E ka arola mefuta e mengata ea manyolo, e leng polar le e senang polar, e sebelisang mefuta e fapaneng ea mekhahlelo e fapaneng le li-solven tsa motlakase joalo ka li-solvents. Karohano e itšetleha ka khethollo e amanang le ea lihlahla tse fapaneng bakeng sa Karolo ea Seteishene, e ka fetoloang molemong oa ho ntlafatsa qeto.

Letsopa, ka lehlakoreng le leng, mekhahlelo ea phapanyetsano ea Iona ea Phapanyetsano ea Li-Aonic ho Khethollo ea Ionic le Metsoako ea polar. Mokhahlelo oa seteishene o entsoe ka li-ion-tsa phapanyetsano ea Ion, e lumella karohano e sebetsang ea li-enions le liahelo. IC e sebelisa li-euents tse telele, hangata li na le metsi a matla a hola kapa acid, mme likarolong tse ngata li etsoa ka mokhoa o ikhethileng ntle le tlhoko ea hoelehetsa.

Litlhahlobo tsa ho sekaseka

HPTC ha e feto-fetoha le maemo a mangata a metsoako ea manyolo, e etsa hore e lokele likopo ho meriana, lijo le tlhahlobo ea tikoloho le tlhahlobo ea tikoloho. Leha ho le joalo, e na le meeli ha ho tluoa linthong tse arohaneng tsa Ionic, joalo ka li-dions le likamohelo tse tloaelehileng tsa HPSc.

IG e hlahisa mefuta e sekaseka setsebi sa iionic le ea ho e etsa mokhoa o ratoang oa ho bona li-auntis tse tloaelehileng Ho bohlokoa haholo masimong a kang ho sekaseka tikolohong ea tikoloho, polokeho ea lijo le phallo ea metsoako ea metsoako e atileng. IC e ka tseba metsoako e seng ea polar polar moreleng le karolo ea mobile e sebelisoang.

U tseba hakae ka li-hippc tsa tlhahlobo le hplc tse lokiselitseng? Ka kopo bala sengoliloeng sena ho ithuta ho eketsehileng:Phapano ke efe pakeng tsa tlhatlhobo ea tlhahlobo le litokisetso tsa lintho tse lokiselitsoeng?

Mekhoa ea ho lemoha

HPTC e tloaetse ho sebelisa li-uv tsa uv, tse hlokang ho hlahlojoa ho monya lero la uv. Sena ke phephetso ea ho bona metsoako e meng, haholo-holo tse sa amobeng leseli la UV, joalo ka li-its tse ling le litsi tsa li-iv le any acid.

IC hangata e sebelisa tlhahiso ea phetoho ea boitšoaro, e sebetsang bakeng sa mefuta ea Iona. Mokhoa o ka fihlela kutloelo-bohloko e phahameng ka mekhoa e kang ea lik'hemik'hale, e fanang ka pherekano ea maemo a morao-rao.

Likopo

HPTC e sebelisoa haholo bakeng sa tlhahlobo ea motsoako o rarahaneng oa manyolo, ho kenyelletsa meralo, lisebelisoa tsa lijo le tsamano tsa tikoloho le tsa tikoloho le tsa tikoloho le tsa tikoloho le tikoloho. Bokhoni ba eona ba ho arola likokoanyana tse ts'oanang bo etsa hore e be sesebelisoa se matla indastering e fapaneng.

IC e molemo haholo likopo tse hlokang tlhahlobo ea mefuta ea Ionic, joalo ka tlhahlobo ea boleng ba metsi, ho hlahlojoa ha lijo le ho hlahlojoa ha phoofolo. Hangata e sebelisoa ho tlatsa HPLC, ho hlokomela bokhoni ba tlhahlobo ea li-laboratori ho kenyelletsa mefuta e mengata ea lihlahla tse pharaletseng tsa lihlahlobo tsa ionic le polar.

Ka kakaretso, khetho pakeng tsa hpl le IG e itšetlehile haholo ka litlhoko tsa tlhahlobo, ho kenyeletsa le mofuta oa metsoako e ithutoang le kutloisiso le tharollo e hlokahalang. Hplce loketse mefuta e fapaneng ea metsoako ea manyolo, leha I IC ke mokhoa oa ho khethoa ha mefuta ea li-ionic le tsa polar.

U tseba mehato ea ho lokisa mekete ea li-chromotography hakae bakeng sa tlhahlobo? Bala sengoloa sena ho ithuta haholoanyane: Mehato e 6 ho lokisa likhamati tsa chromiatography bakeng sa tlhahlobo

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