Tsela eo bo-ralipi ba 1.5ml gc e ntlafatsanang le eona e ntlafatsa mokhoa oa boitokisetso ba mohlala

Moralo oa li-chromatography tsa 1mml li bapala karolo ea kelello ea sampole e sebetsang, haholo-holo ho hasppasis. Li-vaels tsena li etselitsoe ka ho khetheha ho ntlafatsa maemo a tlhahlobo ea metsoako e sa sebetseng ea manyolo (li-vocs). Blog ena e tla hlahloba kamoo litšobotsi tsa1.5ml gc vaialis Tlatsetsa litokiso tse sebetsang hantle, li shebile likarolo tse kang khetho ea boikhethelo, litlhaloso tsa molumo, li tiisa mekhoa ea ho lekola.

Bakeng sa tlhaiso-leseling e batsi ka li-viels tsa AutoSompler bakeng sa li-chromatography tsa khase chromatography, sheba sehlooho sena: 2 ml autosampler

Sebopeho sa lintho tse bonahalang

1. Khalase ea pele ea hydrolytic

Karolo e 'ngoe ea likarolo tse ikhethang tsa 1.5ml gc e entsoe ka khalase ea pele ea hydrolytic. Khalase ena e na le lisebelisoa tsa lik'hemik'hale ebile e nyenyefatsa tšebelisano le sengoli.

Meru e tlase ea Adsorsoption: Khalase e alafshoa ho fokotsa ho buella metsoako ea mantlha, ka tsela eo ka tsela eo ka tsela eo ka tsela nyo Leeto lena le boima ha le sebetsa le mehloli e matla ea joalo ka ha e netefatsa hore ho na le masala a khase eseng ho latela masala a maholo.

2. Likhetho tse hlakileng le tsa amber

Ho fumaneha ha mekhabiso e hlakileng le ea amber e lumella bahlahlobisisi ho khetha ho latela litlhoko tsa bona tse khethehileng:

Li-vials tse hlakileng: E loketseng li-sampole tse sa tsotelleng bobebe, litsietsi tsena li bonolo ho hlahloba le ho lekola.

Malinyane a Amber: Liphae tsa Ammer li bohlokoa bakeng sa metsoako e bobebe ea kutlo ho ba sireletsa ho senyeha nakong ea polokelo le ho hlahlojoa.

Litlhaloso tsa molumo

1. Boholo bo lekaneng bakeng sa tlhahlobo ea lihlooho tsa lihlooho

Molumo oa 1.5 ML o tšoaneleha haholo bakeng sa mekhoa ea sampling ea lihlooho:

Mokhahlelo o lekaneng oa khase: Boholo bona bo fana ka chelete e nepahetseng lipakeng tsa molumo oa molumo oa metsi le Handpace, e fella ka mekhahlelo e lekanang ea li-vofs tse pakeng tsa metsi le khase. Molumo o monyane o fokotsa kotsi ea tahlehelo ea mohlala ka lebaka la ho fetoha mouoane kapa hodsoption.

2. Moqomo o tlase oa ho tlatsa

E nang le tlhoko e tlase ea molumo200 μl, li-vielis tsena li sebelisa mokhoa o nepahetseng oa sampole e lekantsoeng:

Bobapiso ba lisebelisoa: Li-laboratories li ka etsa tlhahlobo ntle le ho senya mohlala oa bohlokoa, o li etsang hore li hlahlobe boleng ba boleng bo phahameng kapa bo sa tloaelehang.

Ho netefatsa mechanism

1. Screw cap

Sistimi ea sekoti e sebelisoang ka har'a li-vaver tse 1,5 ml gc tse ntlafatsa sampole ea mohlala:

Tiiso ea Airtight: Moralo ona o netefatsa tiiso e sireletsehileng nakong ea ho hlahlojoa le ho sekaseka tšilafalo le tahlehelo ea likarolo tse sa fetoheng.

2. Septa ea boleng bo holimo

SeptaE sebelisoa ka li-vaels ka tloaelo e entsoe ka silicone \ ptfe lisebelisoa:

Fokotsa tšilafalo: Lisebelisoa tsena li etselitsoe ho fokotsa kotsi ea tšilafatsa ea ho thibela litšila le ho thibela litlhōrō tsa moea linchengsis tsa chromagragraphic. Ba boetse ba lumella ho kenella habonolo ha nale ea lesela le sa reng letho ebile ha e na ho nyenyefatsa nako.

Ho lumellana le mekhoa ea ho sekaseka

1. Ho feto-fetoha ha maemo bakeng sa mekhoa e mengata

Li-vael tsa li-GC tsa 1.5 ml gC ha li lumellane feela ka GC, empa hape le mekhoa e fapaneng ea lihlahlobo:

Likopo tsa GC-MS: Moralo oa eona o tšehetsa tlhahlobo ea khase ea khase ea khase ea khase ea khase ea khase, e etsang sesebelisoa se fetelletseng bakeng sa lipalesa tsa tlhahlobo ea lihlahla.

2. Khokahano le automomplers

Li-vaels tsena li etselitsoe ho sebetsa ka tsela e sa sebetseng hantle le litsamaiso tsa meqathatso:

Ts'ebetso e eketsehileng: Ho tsamaisana le li-autosomplins, ho fokotsa mosebetsi oa matsoho le ho fokotsa phoso ea motho nakong ea ho lokisa le ho hlahlojoa. Boikemisetso bona bo na le molemo haholo bakeng sa laboratori ba bangata ba tlatsetso.

Taolo ea mocheso le equilibration

1. Meano ea equilibad e ntlafalitsoeng

Moralo oa li-vaiali tsa 1,5 ml gc vaial li tsamaisa mehlala e sebetsang:

Mokhooa o I Eanistatic: Liphalletso tsena li ka phetoa ka thata lipakeng tsa mekhahlelo e potlakileng ea metsi le likhase tse tebileng bakeng sa tekanyo e nepahetseng ea meaho ea vuc.

2. Likhetho tsa ho kopanya

Mefuta e meng e thusa ho kopanya kapa ho hlohlelletsa:

Litefiso tsa phetisetso ea ho fetisoa: Ka ho eketsa sebaka nakong ea ho futhumatsa, likarolo tsena li tsamaisana le katleho e potlakileng le ho ntlafatsa ts'ebetso ea mohlala.

Na u tseba phapang lipakeng tsa li-valc vaia ea li-hpc le li-gc tse tona? Lekola sengoloa sena: Phapang ke efe pakeng tsa li-valiat tsa HPLC le li-gc tse litšila?

Sephetho

Bokhabane bo hlokolosi ba 1.5 ml gc vaialisHo ntlafatsa ts'ebetso ea mohlala haholo ho lokisetsoa ka likopo tsa khase tsa khase. Ho tloha kelellong ea lintho tse bonahalang ho latela mekhoa e metle ea bophahamo le mahlaseli a ho qobang, ho ntlafatsa ho ts'epahala le ho nepahala ha liphetho tsa tlhaiso-leseling. Ho lumellana ha bona ka mekhoa e fapaneng ea lihlahla le ho hokahana le lits'ebetso tse ikemetseng tse nang le matla a phallang a phatsimang.