Ho fokotsa litlamorao tsa Adosorption ka li-chromatography tsa khase chromatography (gc)Li-headSpace li-vialspace e bohlokoa ho fumana litholoana tse nepahetseng le tse khothatsang. Ho thehile ho thehwa ho ka lebisa tahlehelo ea mohlala, tšilafalo le ho fapane ha re bala. Blog ena e tla hlahloba maano a mang a ho fokotsa litlamorao tsena, tse shebiloeng ka khetho e boima, ho toba likoiti le tlhaiso-leseling.

U batla ho tseba haholoanyane ka lebaka leo li-vialspace tse sebelisitsoeng ho chromatography ?, Ka kopo sheba liaparo tsena:Hobaneng ha Headspace e sebelisoa li-chromatography? Li-angles

Tlhahiso ea ho utloisisa ho The Hardpace Vifels

Adsorption ke ha mohatla oa limolek'hule oa khase ea khase ea khase e khomaretsoeng bokaholimo ba methapo ea temo kapa likarolo tsa eona. Ka tlhaiso ea lihlooho tsa lihlooho, metsoako e sa sebetseng ea manyolo (li-vocs) ho tsoa ho sampole ho kenella ho khase ho isa khase ka holim'a eona. Leha ho le joalo, haeba metsoako ena e kenella ka marako kapa septum, palo e sa nepahalang le ea ho boloka lintlha tsa data e sa sebelisoeng.

Lintlha tsa bohlokoa tse amang masene

Sebopeho sa lintho tse bonahalang: Mofuta oa thepa o sebelisoang ho lihlooho o matla o na le phello e kholo ho adsorption.Li-vies tsa khalase, Haholo haholo ba entsoeng ka khalase ea borolicate, ba na le thepa e tlase ea Adsoorp protedoption ha e bapisoa le likhahla tsa polasetiki. Sena ke hobane se setle sa bona se boreleli, se setle se fokotsa tšebelisano ea metsoako e mengata.

Sebaka sa bokaholimo: Sebaka se ka holimo ho karolelano ea bophahamo e ka ama hodemo. Li-vials tse nyane kapa li-vaial tse nang le lihlooho tse ngata tsa pelo ea metsi a ka fokotsa monyetla oa ho etsa hore motho a fokotsehe ka har'a li-vocs.

Mocheso le nako: mocheso o eketsehileng o eketsa ho volanit ea sekaseka, empa hape e ka ntlafatsa alesereishene haeba thepa ea vae e sa tšoanele lithemperateng tse phahameng. Ho feta moo, linako tse telele tse betliloeng li ka fana ka nako e ngata ea ho boleloa ho etsahala.

Batla ho tseba tsebiso e felletseng mabapi le li-heltiespace vial, ka kopo sheba sengoloa sena: Tataiso e Phethahetseng ea ho Rulōla Peespace e Hlophisang: Likahaka, khetho, theko le ts'ebeliso

Maano a ho fokotsa litlamorao tsa adssorption

1. Khetha thepa e nepahetseng

Ho khetha li-vael tsa khalase tsa boleng bo holimo ho bohlokoa ho fokotsa litlamorao tsa Adssorption. Khalase ea borolicate e khothalletsoa ka lebaka la ho hanyetsa ka lik'hemik'hale ea eona ea lik'hemik'hale le tšebelisano e tlase le li-vocs. Haeba li-vavers tsa polasetiki lia hlokahala, etsa bonnete ba hore li entsoe ho tsoa ho phahameng-dencelene

2. Fana ka mohlala oa ho itokisa ha mehlala

Ho itokisa ka mokhoa o nepahetseng ho ka fokotsa monyetla oa ho etsa Kreste:

Mohlala oa mohlala: Etsa bonnete ba hore ke mohlala oa mokelikeli o lulang 10-50% ea molumo oa vaerate. Karolo ena e re thusa ho boloka lihlooho tse lekaneng ha u ntse u ikopanya le metsi le marako a maholo.

Ts'ebeliso ea Dinrivatization: Ho feto-fetoha ho ka fetola litlhahlobo tse sa tloaelehang pele ho tlhahlobo, ka hona, adsorigity le ho fokotsa matla. Mohato ona o na le thuso haholo bakeng sa metsoako e tloaetseng ho adsorb ho isa libakeng.

Mehopolo ea Matrix: Ela hloko sebopeho sa mohlala oa Matrix. Boikutlo bo phahameng ba bilicular kapa sampole ea li-viscous li ka hloka linako tse telele kapa mekhoa e ikhethileng ea ho fokotsa masala a ka bang le masala a maholo.

3. Tsamaea maemo a tikoloho

Ho tsamaisa Lintho tsa Tikoloho nakong ea sampleling ho ka thusa ho thetsoa ha maele:
Taolo ea mocheso: Boloka mocheso o sa khaotseng nakong ea ho lekana le ho sekaseka. Qoba mocheso o feteletseng, o ka eketsang khatello ea mouoane 'me u etella pele ho sampling kapa ho nyenyefatsa metsoako e sa tsotelleng.

Fokotsa molumo o shoeleng: Ho etsa bonnete ba hore lintho tsohle tse amanang le sampless li etsoa hampe ho fokotsa molumo, moo livaors li ka iteroselang kapa livaorb pele li fihla kholomong ea GC.

4. Ho kenya tšebetsong mekhoa e nepahetseng ea mohlala

Mokhoa oa ho fetisetsa Hlooho ea lihlooho e ka ama adsorption:

Use an Autosampler: An automated headspace sampler provides consistent pressure and time during sampling, helping to minimize variability caused by manual handling.

Mekhoa ea ho hatella: Etsa kopo ea ho hatella ha mafura pele ho latela mohlala oa ho kopanya le ho fokotsa kotsi ea ho lahleheloa ke ho phokaLeshano. Ho lieha ho khuts'oane kamora ho tobetsa ho lumella khase hantle ea khase ka har'a khase.

5. Tlhokomelo e tloaelehileng le calibration

Mokhoa oa ho hlokomela tsamaiso ea GC le ho leka-lekanya sesebelisoa ho ka thibela mathata a amanang le tšilafalo le ho netefatsa sephetho se nepahetseng:

Lekola Bothata ba Septum: Netefatsa hore Septum e sebelisitsoeng ka temoso e tsamaisana le tiiso e ntle e ke ke ea tsoa ho tiiso e ntle ea hlooho. Nahana ka ho sebelisa Septa-lipta bakeng sa li-solvent tsa manyolo.

Seteishene sa ts'ebetso ea ho hlophisa: Hloekisa mohala oa phetisetso le likarolo tsa GC khafetsa ho thibela litlhoro tse sa batleheng ho tsoa litlhoro tse sa batleheng chromatogram.

U batla ho tseba ho hloekisa Headspacespace ea Groromatography? Lekola sengoloa sena:Mokhoa oa ho hloekisa lihlooho tsa likhomo tsa chromanigraphy?

Sephetho

Fokotsa litlamorao tsa Adosorption hoGC headpace vialsHo hloka mokhoa o felletseng o kenyelletsang ho khetha thepa e loketseng, ho laola maemo a tikoloho, le ho boloka lisebelisoa. Ka ho latela lintlha tsena, bahlahlobi ba ka ntlafatsa tsebo e tšepahalang le ho nepahala ha lits'ebetso tse fapaneng ho ea ho taolo ea tikoloho lits'ebetsong tsa boleng.

E fetileng:

1.5ml vaials bakeng sa li-chromatography tsa chromatography (LC)

E 'ngoe:

Tsela eo bo-ralipi ba 1.5ml gc e ntlafatsanang le eona e ntlafatsa mokhoa oa boitokisetso ba mohlala