Mekhoa ea li-chrotography ke ea bohlokoa masimong a fapaneng a saense, tlhahlobo ea mekhabiso e rarahaneng. Leha ho le joalo, har'a metsamao le tlhokomelo ea kelello ho qaqisa, e le tšokelo e bohlale empa e le sokela e lubola ka har'a litlhophiso tsa laboratoring:Malinyane a chrotographylitšila. Lijana tsena tse neng li bonahala li ka etsahala, li le bohlokoa ho disampole tsa matlo, li angoa ke mefuta e mengata e ka sekisang botsitso ba liteko tsa liteko. Sehloohong sena, re ithuta ho ba karolo e mengata ea litlamorao tse nang le ciafotols, re sheba tšimoloho ea eona ea Chromatography, re tšoaeang tšimoloho ea eona.

1. Mehloli ea likhau tsa chromatography

Ho tšoaea liphoso tsa li-chromamagraphy mehloling ea li-chromande tse tsoang ho li-mmardad tse ngata, e 'ngoe le e' ngoe e boemong bo ikhethang ba ho etsa boiteko bo ikhethang:

Likhah tsa Kantle:Tikoloho ea laboratori, leha ho leka ho boloka ho boloka moetlo, ho jarolla mahlahatso, masalla a liteko le li-actimal. Ho silafatsa litšila tsena ka ntle ho li-cumatography ka har'a maqhubu a moea kapa puisano e tobileng ka kotloloho, hlahisa litšila tse sa batleheng hore li be mehlala.

Likalimo tsa kahare:Ho makatsang ke hore litabeng tsa bona li ka sebetsa e le batho ba litšila bakeng sa tšilafalo. Lintho tse setseng ho sebelisoa ka lisebelisoa tsa pele, kapa ho se phethahale ka har'a khalase ho ka baka bohloeki.

Li-laps tsa tsamaiso:Phoso ea motho e lula e le mothusi ea bohlokoa ho ea ho khato ea likhama tsa charomamography. Ho kheloha ho tsoa ho li-protocol tse thehiloeng ka mokhoa oa ho tsamaisa, ho boloka kapa ho itokisa lekhetho ho ka kenya ts'ebetsong ea ho tšepahala ha litholoana tsa tlhahlobo.

Lekola lits'ebeliso tse 15. Delve ka ho fokola le bohlokoa ho feta masimong a mahlale a fapaneng.Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

2. Kameho ea litšila tsa likhahla tsa ciaromatography ka liphetho tsa liteko

Liphello tsaMalinyane a chrotographyTs'usumetso e khutlisetsang ts'ebetsong ea mahlale, ho lahla ho belaela ha liphutuloana:

Liphello tsa liteko tsa liteko:Lintjaro, ebang ke lik'hemik'hale, tse kang tsa tlhaho kapa tsa tlhaho, li ka sebelisana le litsebo tse sa lebelloang ebe li lebisa liphatlalatso kapa boits'oaro.

Ho hlahisoa tlhahiso:Ho ba teng ha li silakanya ho hlahisa ho se tšoanelehe le ho sa lebelloa ho pheta ka liteko tsa liteko, ho thata ho e etsa. Ho se lumellane ho li-e li qoqileng ho nyenyefatsa tumello ea linako tse fumanoeng li-chrotographic lihlahlobisisi.

Sesebelisoa sa Sesebelisoa:Ho kenyelletsa litholoana tse matlafatsang, tšilafalo e kholo e beha kotsi e bonahalang ho clomatographphic. Tekanyetso ea li-killints tse entsoeng ka mekhabiso e metle joalo ka likholomo kapa li-disctor li ka fokotsoa ka mokhoa o boima, li fumana litokiso kapa likoloto tse theko e boima.

Fumana mehato ea bohlokoa ea ho itokisa hantle ea limpho tsa khalemelo e nepahetseng, e bolela ka nepo le ho itlhahloba ha ho na bopaki. Master mokhoa oa mehato e tšeletseng feela e bonolo!Mehato e 6 ho lokisa likhamati tsa chromiatography bakeng sa tlhahlobo

3. Thibelo le mekhoa ea ho tlohela

Ho fokotsa kotsi ea khahlano ea likhamante tsa ciaroma perfic e batla mokhoa o phethahetseng o hlokang mehato e phethahetseng e kenyelletsang mehato e nang le mokhoa o ikhethileng le ho kenella ha likarabo:

Li-protoche tsa li-strictant tsa laboratori:Ho hloekisa le ho tšoaea liphoso tsa laboratori, lisebelisoa, lisebelisoa tsa moea li fokotsa ho ata ha li-canmaticing tsa chromatography le li-disap.

Tloaelo e tloaelehileng ea tlhatlhobo le tlhokomelo:Litlhahlobo tsa nako le nako tsa bohloeki le botšepehi li etsa hore ho se khone ho lemoha ka botsitso kapa ho senya. Liphetoho tse potlakileng kapa mekhoa ea ho hloekisa ka mokhoa o felletseng e fokotsa kotsi ea liphoso tse hlahisoang ke litsiehi.

Ho latela melao e tšoanang:Ho theha mananeo a ho koetlisa le ho khomarela li-protocol ha ho bohlokoa ho fokotsa tšilafalo e susumetsang batho. Tataiso e Hlakileng Bakeng sa Boitokisetso ba Mohlala, Leetong la Boitokiso, 'me ho boloka ho khothaletsa Constenc le sekaseka lihlahlobisisi tsa chromatographic.

Tlhahiso ea lisebelisoa tse netefalitsoeng:Ho tsetela ho li-vaelmatograph tsa boleng bo holimo ho li-chromagraphy tse entsoeng ho tloha kahare ho thepa ho fokotsa monyetla oa hore o na le monyetla oa ho tšepahala kapa ho tšepahala ho tlohelang mohlala.

Sephetho

Malinyane a chrotographyHo tšepahala ho hong ho teng ho tšepahala le tšehiseli ea ho tšepahala le tšehiseli ea sephetho sa sephetho. Ka ho lemoha mehloli e fapaneng ea tšiea, e utloisisa litlamorao tsa eona tsa tlhahlobo, li-laboratories li ka matlafatsa lits'ebetso tsa tsona tsa setsebi khahlanong le kotsi ena e bolotsana e khopisang. Ka ho pele ho ho bolokoa ha saense ea bohloeki, bafuputsi ba hlahisa lihlahlobo tse nepahetseng, tse entsoeng ka boomo le morero.

Bula likarabo tsa lipotso tse 50 mabapi le li-valc vaialis tse ngotsoeng sehloohong sena se felletseng. Fumana leseli ka mefuta ea val, ts'ebeliso le tlhokomelo: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Cap e tlamang ke eng?

E 'ngoe:

Ho sebetsana le maemo a ho kena-kenana le li-chromatography