Mohloli oa mohlala o na le tšusumetso e kholo. E ama ts'ebetso le liphetho tsa chromatography. Haeba sampole e sa tloaelehang e hloekisitsoe, e tla u silafatsa. Ha Khabatseno ena e kanna ea baka: Litlhōrō tsa moea, mohlala, mohlala, maikutlo, bofokoli bo fosahetseng, tse phehellang, tse phehellang le litlhōrō tse bakoang ke ba bang. Ho sebetsoa ka mokhoa o sa tšoaeoang hampe le tla ama liphetho tsa tlhahlobo haholo. Sena se tla baka ho kheloha liphethong esita le ho senya autosampler.

Kahoo, ho hloekisaVososampler Viae bohlokoa haholo. Teko ea setheo e tlameha ho tseba mokhoa oa ho hloekisa oa Val.

Batla ho tseba ho tseba ka lintlha tse mabapi le li-valc vaiale, ka kopo sheba sengoloa sena: Encyclopedia of Hplc vaialis

Ho joang

Mokhoa oa ho hlatsoa o fapane ho latela tekanyo ea tšilafalo ea masela. Sengoliloeng sena se tla buella lintho tse ngata tsa ho hloekisa tse ngata. Li tla u thusa ho qeta tlhahlobo ea li-chrotographicgraphicgraphicphic.

Mokhoa oa 1

1. Tšela tharollo ea liteko hoThe HPLC Vial.

2. Commese e sa tloaelehang ho 95%, ea hlola e hloekisitse habeli, ebe e tšela joala (ho tsepamisa lino tse ngata haholo.

3. Tšela ka metsing a hloekileng mme ka mokhoa o hloekileng o le hloekisa habeli.

4. Tšela mokelikeli o hlatsoang ka holong o tenang 'me o baka celsius ka lihora tse 1-2 (se ke oa baka mocheso o phahameng, oo o tla baka tšenyo ea vaisa.

5. Pholile le ho boloka.

Mokhoa oa 2

1.Rese makhetlo a mangata ka metsi a pompo.

2. E e beha ka beaker ea metsi a khelohileng le ho sebelisa sesepa sa ultrasonic metsotso e leshome le metso e mehlano.

3. Fetola metsi ebe u mo fa metsotso e 'ngoe e leshome le metso e mehlano ea ho hloekisa ultrasonic.

4. Tšela e le 'ngoe e ngata haholo ka beneker e monate e nang le monate.

5. Qetellong, e tlose 'me u e tlohele ka tlhaho ka moea.

Mokhoa oa 3

1. E a qhekella metsotso e mashome a mabeli, ebe o tlosa metathano ebe o e hloekisa ka tsaletrasonographgraphy.

2. Kamora ho eketsa metsi a mangata ho sampole ea boima ba mohlala, ultrason e hloekise metsotso e 20 ebe ebe e hula metsi.

3. LumellaLeshanoho omella.

Mokhoa oa 4

1. E omme le metsi

2. Soak seriri ka lihora tse 4, ka mokhoa o hloekileng bakeng sa halofo ea hora, ebe o hloekisa metsi halofo ea metsi ebe o omella.

Mokhoa oa 5

1. Soak ka moemeli ea matla ho hloekisa ka lihora tse 24.

2. Hloekile makhetlo a 3 ka metsi a make ka tlas'a maemo a ulfasonic, 'me qetellong a hloekile hang ka methanol,' me a e omelle hore e sebelisoe.

Mokhoa oa 6

Sebelisa mochini oa ho hlatsoa mochini. Mochine oa ho hlatsoa botlolo o khona ho ntlafatsa ts'ebetso ea likhama tsa ho hloekisa le ho boloka basebetsi le nako.

Na u tseba ho hloekisa likhama tsa hau tsa HPPC? Lekola sengoloa sena: Mekhoa e mehlano ea ho hloekisa bakeng sa li-valc vaials

Mathata ao u ka kopanang le ona

Ho na le mathata a mangata a hloekisitsoeng ka matsoho.

1. Ts'ebetso e tlase ea ho hloekisa, nako e telele ea ho hlatsuoa, ho mamella le ho etsa boiteko. Ntle le moo, palo ea borashe le li-sekhoutu li thata, li thata ho li laola, mme li senya lisebelisoa tsa metsi.

2. Ho hloekisa ho thata ho likhutlo tse shoeleng, ho thata ho tiisetsa hore ho ka hloekisa litholoana, ho ama sephetho sa liteko, le litšenyehelo tsa thepa le tse ngata.

3. Ho na le likotsi tsa polokeho ts'ebetsong ea ho hloekisa ea basebetsi ba hloahloa. Li-laboratori tse ling li tla sebelisa mochini oa ho hloekisa oa ultrasoc. Mokhoa ona oa ho hloekisa o ntlafatsa ts'ebetso ea ho hloekisa le bohloeki ho isa tekanyong e itseng, empa e na le mathata.

Mochine oa ho hloekisa oa ultrasoc o lerata ebile o le thata nakong ea ts'ebetso ea ho sebetsa, 'me ho bonolo ho tlohela temoso,' me nako ea ho hloekisa ha ho bonolo ho e utloisisa. Ka hona, re khothaletsa hore o tsamaisa liatlana, masela, appgles le lisebelisoa tse ling tsa polokeho nakong ea ho hloekisa.

Haeba e le motho ea moholo kapa ea senyehileng, u se ke oa e sebelisa hape. Ho ipapisitse le hore na u etsa liteko hangata hakae, nka sebakaMohlala o Tebilenglikhoeli tse 1-3.

Liphallelo tse lanngoeng li hloka ho laoloa ka mokhoa o ts'oanang, li sebetsoa ho latela melao ea ts'ehetso ea laboratori, 'me e ne e le lik'hamphani tsa kalafo tse sa tšoaneleheng.

Mokhoa o mong le o mong oa ho hloekisa o na le menyetla e lumellanang le mathata. Mokhoa o motle ka ho fetisisa ke ho nkela ntho e ncha ka mor'a tlhahlobo ka 'ngoe. Ho bohlokoa hore o khethe mokhoa o nepahetseng oa ho hloekisa. Ho ipapisitsoe le mofuta oa sampole ea tlhahlobo le maemo a nnete a laboratori, khetho ena e lokela ho etsoa. Ho boetse ho khothalletsoa ho nahana ka litšenyehelo tsa nyeoe. Re lokela ho hopoloa hore ho latela tlhahlobo e 'ngoe le e' ngoe ea li-chromaticgraphic, Septum oa vaelo ea mohlala o hloka ho nkeloa sebaka.

Wan Ho Tseba Tsebo e Feletsoeng mabapi le Tsela ea ho Hloekisa Liphatlalatso tsa Chromatography, ka kopo sheba sengoloa sena: Ka ho sebetsa hantle! Mekhoa e 5 ea ho hloekisa li-chromatography tsa mohlala oa mohlala

E fetileng:

Khetha li-pilf tse ntle tsa kalafo ea HPTC ho ntlafatsa tlhahlobo ea hau ea HPST

E 'ngoe:

Na u utloisisa taolo ea boleng ba li-hipc li-vials?