Lihlahisoa tsa lipalesa tsa hlooho ea hlooho ea li-chromatography Mokhoa o lekantsoeng ka mokhoa o sa tloaelehang ho sekaseka magrame o rarahaneng ntle le ho kenya sampole eohle ka kotloloho ho chromatograph ea khase (gC). Ho fapana le moo, hs-gc e hlahloba karolo ea khase ka holim'a sampole, e tsejoang e le hlooho ea hlooho. Blog ena e tla koahela tsohle tseo o hlokang ho li fumana ka mekhabiso ea hlooho ea hlooho, ho kenyelletsa le melao-motheo ea eona, mekhoa, melemo le mekhoa e metle.

Batla ho tseba tsebiso e mabapi le litaba tsa hlooho ea lihlahisoa, ka kopo sheba sengoloa sena: Tataiso e Phethahetseng ea ho Rulōla Peespace e Hlophisang: Likahaka, khetho, theko le ts'ebeliso

Plempace samepling

Sampling e thehiloe molaong o boima ba hore metsoako e thata ka ho fetisisa e ka arola mohlala oa ho hlonepha ha ho futhumatsa kapa ho lekana. Mokhoa ona o bohlokoa haholo ho sekaseka metsoako ea metsoako e sa sebetseng ea manyolo (Vocs), li-solves tse setseng le lintho tse ling tse ngata tse phehang. Mekhoa ea lihlooho e nyenyefatsa ho hlahisoa ha likarolo tse sa fetoheng le litšila tsa GC, li fella ka li-chromaner le litholoana tse tšepahalang haholoanyane.

Mohopolo oa bohlokoa oa sampling ea lihlooho

Equilibrium:Ha mohlala e kenngoa ka har'a Leliba le tiisitsoeng Metsoako e futhumetseng, metsoako e matla e tla falla ho tloha moketeng kapa karolo e tiileng ho fihlela karolo ea khase ho fihlela e lekanang. Khatello ea taba ea sekaseka hloohong ea lihlooho li itšetlehile ka ho ba le maemo a phahameng le mocheso oa sampole.

Karolo ea karohano (k):Karolelano ea Tekanyetso ea Tefo ke ntho ea bohlokoa e hlophisitsoeng ea bohlooho. E hlalosa kabo ea metsoako e sa fetoheng lipakeng tsa mekhahlelo ea metsi le khase. Metsoako e nang le li-apole tse tlase tsa k e tlase ka ho arola habonolo ka mohato oa khase mme ka hona a fumanoang habonolo.

Static vs. Rynamic Headpace:Sampling sampling sampling e ka etsoa ho sebelisa mekhoa e matla kapa e matla. Lihlahisoa tsa Sperics se kenyelletsa ho lumella sampole ho fihlela sampole ho ea semmuso ka lebaka la khase e matla ho fiela metsoako e sa fetoheng mohatong oa khase.

Li-HeadPeses ea li-headpamolete ea li-chromatography

Litokisetso tse nepahetseng tsa sampole li bohlokoa ho fumana litholoana tse nepahetseng le tse khothatsang ho litholoana tsa likhose tsa likhoho tsa likhose tsa likhose. Mehato e latelang e bontša litekanyo tsa mantlha tsa ho itokisetsa mekmale bakeng sa tlhahlobo ea lihlooho tsa libuka.

1. Khetha sampole e nepahetseng

Ho khetha ka nepomohlalae bohlokoa bakeng sa mehlala e atlehileng ea hlooho. E tloaelehileng e tloaelehileng e kenyelletsa 6ml, 10ml le 20ml, le20ml vaialsho ba tse sebelisoang haholo. Lintlha tsa Bohlokoa Tseo U Lokelang ho nahana ha li khetha ho khetha:

Lintho tse bonahalang:Li-vaes li entsoe ka khalase kapa polasetiki.Li-vies tsa khalaseba tšoaneloa hamonate hantle bakeng sa mehlala e sa tloaelehang ka lebaka la ho bopa ka mokhoa o makatsang le ka bokhoni bo tlase ba lesholu le silafetseng.

Ho tiisa Mechanism:Li-viello li ka tiisoa ka crimp kapa li-cap tsa screw. Li-vaiali tsa crimpFana ka tiiso ea mahlo, e leng ntho ea bohlokoa ho boloka botšepehi ba hlooho ea lihlooho.

Boleng ba Septum:Septa e ne e tloaetse ho tiisa li-vaels tse ka hlahisang singlents haeba li sa boleng bo bobe. Batla Septa e thetsitsoeng ka ho khetheha bakeng sa lits'ebetso tsa lihlooho, kaha li na le monyetla oa ho ea kena hloohong ea hlooho.

2. Mohlala oa mohlala.

Bophahamo ba mohlala ka vaerase ho bohlokoa ho fihlela ho fihlela lihlooho tsa lihlooho tsa hlooho e nepahetseng. Ka kakaretso, molumo oa mohlala o lokela ho ba ho 1 \ 3 ho 1 \ 2 ho 1 \ 2 of the molumo o lekaneng oa Val.

Tšebeliso:Haeba samboto ea mantlha e phahame haholo, e ka baka hlooho e kholo haholo, e lebisang ho tse sa nepahalang. Ho hlapolla sampole ka solvent e loketseng ho ka thusa ho fihlela maikutlo a lakatsehang a sekaseka.

U batla ho tseba haholoanyane ka lebaka leo li-vialspace tse sebelisitsoeng ho chromatography ?, Ka kopo sheba liaparo tsena: Hobaneng ha Headspace e sebelisoa li-chromatography? Li-angles

3. Taolo ea mocheso

Mocheso o bapala karolo ea bohlokoa ho mosali oa hlooho ea hlooho ea hlooho ea khomo ha e ama ho ba hlahlojoa le ho lekola ha bona mohatong oa khase.

Mocheso oa tekano:Li-daiala tsa sampole li lokela ho futhumala mochesong o laoloang ho khothaletsa ho lokolloa ha metsoako e litšila hloohong. Mocheso o nepahetseng o ipapisitse le sekaseka se ikhethileng se hlahlojoang 'me o lokela ho khethoa nakong ea ts'ebetso ea mokhoa.

Nako ea tekano:Fana ka nako e lekaneng ea mohlala ho fihlela tekano. Sena se ka fapana ho latela mohlala mattrix le ho vouta ka metsoako ena. Linako tse tloaelehileng tse lekanang ho tloha metsotso e 30 ho isa ho lihora tse 'maloa.

4. Fokotsa litšila

Ho silafatsoa ho ka ama ho nepahala ha lihlooho tsa lihlooho tsa hlooho ea li-chomatograph tsa hlooho tsa khomo Ho fokotsa kotsi ea tšilafalo, etsa tse latelang:

Sebelisa li-vials tse halikiloeng pele:Kamehla sebelisa li-valis tse halikiloeng esale pele ho qoba kenyelletso ea lithibelo nakong ea ho paka kapa ho sebetsana le tsona.

Likheo tsa Metho:Run Mehato ea Mehato ea ho tseba mehloli ea tšilafalo. Sena se kenyelletsa ho hlahloba sampole e se nang letho ho sebelisa lisebelisoa tse se nang letho le lits'ebetso tsa thuto ho netefatsa hore ha ho litlhoro tse sa batleheng chromatogram.

Maemo a laolehileng:Etsa litokisetso tsa mohlala tikolohong e hloekileng ho fokotsa ho pepesetsoa litsing tsa moea.

5. Khetha mokhoa o nepahetseng oa lihlooho

Joalokaha ho boletsoe pejana, lebitso la HeadPace le ka ba matla kapa le matla. Khetho ea mokhoa oa mokhoa o itšetlehileng ka tšebeliso e khethehileng le mofuta oa sampole.

Lihlooho tsa Stretic:Mokhoa ona o loketse likopo tse ngata 'me o sebelisetsoa hohle ho sekaseka metsoako e litšila lino le li-solarsids. E lumella tlhahlobo ea tlhaiso-leseling ka tlhaho ntle le ho hlahisoa ha khase e eketsehileng.

Rnanamic Headpace:Mokhoa ona o tšoaneloa ke disampole tse hlokang pontšo e tsoelang pele ho hapa metsoako e sa tsotelleng. Hangata ho sebelisoa lits'ebetsong tse kang tlhahlobo ea tikoloho le tlhahlobo ea lijo moo mehlala e nang le likhomo tse tlase tsa lihlahlo li ka bang le eona.

Melemo ea lihlooho tsa SOWSPACE

Choromanegraphs:Ka ho sekaseka feela karolo ea mouoane, Samppace Samptiling e fokotsa ho hlahisoa ha likarolo tse sa fetoheng le litsomi, li lebisang li-chromanegrams le tlhahlobo e ntlafalitsoeng.

Nako e Fokotseng Nako ea ho lokisa:Sampling Depliling e nolofatsang ts'ebetso ea sampole, kaha e felisa tlhokahalo ea lits'ebetso tse rarahaneng kapa lits'ebetso tsa bohahlauli.

Keketseho e eketsehileng:Ho tsepamisa mohopolo ka mokhoa o ikhethileng ho lihlooho ho ka etsa hore ho be bonolo ho ntlafatsa matla, ho etsa hore ho be bonolo ho bona metsoako e mengata ea traces.

Ho fapanyetsana:Sampling sampling e ka sebelisoa libakeng tse ngata tsa mohlala, ho kenyelletsa lino, li-solar, tse rarahaneng, tse rarahaneng, li etsa hore ke mokhoa o sa fetohaleng lits'ebetsong tse fapaneng.

Batla ho tseba haholoanyane ka tekanyetso ea khatello ea li-aijugh Stress le li-caps, ka kopo sheba liaparo tsena: Tekanyetso ea khatello ea li-ijiren ke ea li-vielis le li-caps tse kae?

Sephetho

Li-headpatographs tsa mafurake mokhoa oa bohlokoa oa tlhahlobo oa tlhahlobo ea likhakanyo tse bobebe tsa mehlala e fapaneng ea mohlala. Ho ikhopolla ka mokhoa o nepahetseng hoa bohlokoa ho fihlela ho fella ka ho nepahala ho nepahetseng le ho hlahisa li-con ho se tsotelle. Ka ho utloisisa melao-motheo ea samonte ea hlooho le ho latela mekhoa e metle bakeng sa boitokisetso, li-laeboratories li ka ntlafatsa ts'ebetso le ho ts'epahala ha litlhahlobo tsa bona. Ha mekhoa ea boleng bo phahameng ea tlhahlobo e phahameng e ntse e tsoela pele ho hola, Lipeo tsa Spectpace GC li tla lula li le sesebelisoa sa bohlokoa bakeng sa likhakanyo le bahlahlobising tse ngata.

E fetileng:

HPTC v. LCMS: U lokela ho khetha mang? Lintlha tse 5

E 'ngoe:

Mokhoa oa ho Sebelisa Liaparo tsa Cod ka nepo bakeng sa tlhahlobo ea boleng ba metsi?