HPTC v. LCMS: U lokela ho khetha mang? Lintlha tse 5

Molao-motheo oa Ts'ebetso

Hplc

HPTC ke mokhoa oa chromatographic o arohanang ka metsoako e thehiloeng mesebetsing ea bona le mekhahlelo ea maemo le mobile. Ho HPLC, karolo ea mobile ea mobile e pommetsoe holima karolo e tlalehiloeng ka karolo ea setera, eo hangata e entsoeng ka likaroloana tse nyane tsa Sina kapa tsa polymer. Ha motsoako oa mohlala o kenngoa ts'ebetsong, likarolo li arohantsoe ho latela mohato oa ho ema. Metsoako e sebelisanang ka matla le mokhahlelo oa boemo bo phahameng butle butle, ha metsoako e 'ngoe e sebelisana haholo kapele haholo. Metsoako e arotsoeng e fumaneha ka ho sebelisa li-disctor tse fapaneng, tse kang UV, tse tummeng kapa li-dister tsa index.

Batla ho tseba haholoanyane ka lits'ebetso tsa li-varomatography tsa chromatography, ka kopo sheba sengoloa sena:Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

Lcms

Li-LCM li kopanya bokhoni ba karohano ea HPLC e nang le bokhoni ba ho itlhahloba tsa bahlale ba litaba tsa boima ba naha. Ka LCMS, lisamona li arohantsoe le HPLC, feela joalo ka li-HPLC. Leha ho le joalo, ho e-na le ho fumanoa ke "iphatep ea tloaelehileng, mekhahlelo e mengata e kentsoeng e hlahisoa ka pono e kholo. Bo-'mōtoana bo boholo bo itšilafatsang likotloana le ho lekanya tekanyo ea bona ea ho beha ka bongata, ho fana ka leseli le qaqileng ka boima ba limolek'hule le sebopeho sa sekaseka. Motsoako ona oa karohano le batho ba bangata ba e-ba le kutloelo-bohloko o fana ka maikutlo a maholo le ho hlaka ho feta HPLCE feela.

Mechanism ea karohano

HPTC:HPLC e sebelisa khatello e phahameng ea ho sutumetsa karolo ea mobile ea mobile ka holim'a kholomo e nang le tšibollo e nang le karolo ea boemo bo phahameng. Metsoako e arotsoe ho latela tšebelisano le 'ne e fapaneng le mohato oa boemo bo holimo, e fella ka karohano e hlakileng le e sebetsang.

Lcms:Li-LCMS li kopanya bokhoni ba karohano ea ho arohana ea 'mele ba HPTC ka bokhoni ba' mele bo boholo ba li-capper tse ngata tsa Spearcrometry (ms). Karolo ea chromatography e arola likhamphani, ha litaba tse ngata tsa bona tsa pono e lekanya tekanyo ea mefuta e mengata ea liqoso.

Kutloisiso le khethollo

HPTC:HPTC e na le maikutlo a matle le temoso, haholo-holo ha a kopantsoe le malumeli a totobetseng a kang UV Leha ho le joalo, HPLC e le 'ngoe e kanna ea se fane ka maikutlo a lekaneng bakeng sa disampole tse thata.

Lcms:Li-LCMS e na le maikutlo a phahameng le khethollo e bapisoang le hplc. Bongata ba lihlomo ba ka khetholla ka nepo le ho tiisa metsoako e thehiloeng karolong e ikhethang ea ho lefella, leha e le match e rarahaneng. Sena se etsa hore LCMS e be thuso haholo bakeng sa ho hlahloba metsoako e bolaeang le ho netefatsa boitsebahatso ba lintho tse sa tsejoeng.

HPTC:Mohlala oa sampole o tobane le HPLC o kenyelletsa ho ts'oara, kapa mekhoa e bonolo ea ho tsoa. Sampole ha ho hlokahale hore ho senyeha, kahoo HPLC e loketse metsoako e mengata, ho kenyeletsoa lintho tse kholo le tse seng tse seng tse seng tse sa sebetseng.

Lcms:Litokisetso tsa samms li tšoana le bakeng sa HPLC, empa mehato e meng e ka hlokahala ho netefatsa hore ho lumellana le pono ea Bohata. Mohlala, li-buffger kapa li-busttive li ka hlokahala ho ntlafatsa ionization le ho thibela khatello ea Ion.

Likopo

HPTC:HPTC e sebelisoa haholo bakeng sa tlhahlobo ea meriana, lijo le sampole tsa tikoloho. Ho sebetsa haholo bakeng sa ho arola metsoako e kang ho sebelisa lithethefatsi, joalo ka litšila tsa mafu a li rarahaneng, tse ling tsa masala a lijo.

Lcms:Li-LCMS e na le likopo tse fapaneng tsa likopo, ho kenyelletsa:

Tlhahlobo ea phamasike: e sebelisoang ho likololelo ea lithethefatsi, nts'etsopele le taolo ea boleng.

Lithepumpikis le metabolomics: tse sebelisoang ho khetholla le ho khetholla liprotheine le metabotolites ho tsa bioloji.

Tlhahlobo ea tikoloho: e ne e bona ho bona litšila tsa sincrant ka metsing, mobu le sammanane ea moea.

O labalabela ho ba le karolo ea li-hilc tse netefatsang tsa HPTC ho netefatsa tlhahlobo e nepahetseng ea mohlala? Bala sengoloa sena: Bohlokoa ba li-vialc tse netefalitsoeng tsa HPSC bakeng sa tlhahlobo e nepahetseng ea mohlala

Sephetho

HPTC le li-lcms ke mekhoa e metle ea tlhahlobo e matla ka matla le mefokolo ea bona. Khetho pakeng tsa tse peli ho latela litlhoko tse ikhethileng tsa tlhahlobo, joalo ka boiketlo, ho khethoa, ho rarahana le lisebelisoa tse teng.

HPLC ke khetho e ntle bakeng sa tlhahlobo e tloaelehileng ea metsoako e tsebahalang ea matric e bonolo, moo litšenyehelo le boiketlo ba bohlokoa ke lintlha tsa bohlokoa. Ka lehlakoreng le leng, boloi bo ratoang ba ho sekaseka li-sampole tse rarahaneng, ho supa metsoako e sa tsejoeng.

Ha theknoloji e ntse e tsoela pele, 'me li-LCMS li tla bapala karolo ea bohlokoa ea ho fetola lequestrical le ho netefatsa sephetho se phahameng.