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Mokhoa oa ho Boloka hantle le ho sebetsana le liphaephe tse netefalitsoeng tsa chromatography

Awar. 18th, 2024
Litags: Li-chromatographs-Valis Li-chromatography-Valis Vias-chromatography-2l 2ml-hplc-vaal li-khalase tsa likhalase 2ml-hplc-vial-with-cap 13mm-hplc-vials 2ml-hplc-vialcs
Litomana tsa li-chrotography ke sesebelisoa sa bohlokoa ho khethollo ea tlhahlobo ea tlhaiso-leseling le ho bapala karolo ea bohlokoa ho polokelo le ho hlahloba mehlala. Ho boloka hantle le ho sebetsana ka mokhoa o ikhethileng ho boloka ho hloka toka le ho nepahala ha likhama tsena. Sengoliloeng sena se re temana ea bohlokoa bakeng sa polokelo le ho sebetsana le litakatso tsa chromagraphy tse tiisitsoeng tsa chromatography ho netefatsa ts'ebetso e nepahetseng le liphetho tse tšepahalang.

Mabapi le li-canromatography

Lithako tsa ChromatograftSebeletsa e le sejana sa bohlokoa sa ho boloka mehlala ka k'hemistri ea ntlha ea ho sekaseka. Li fumaneha ka lisebelisoa tse fapaneng, ho kenyelletsa le khalase le polasetiki, ho fana ka melemo e 'ngoe le e' ngoe e fapaneng ho latela mofuta oa tlhahlobo ea tlhahlobo le sebopeho sa sampole. Li-vaels tsa khalase li sebelisoa hangata hobane li ka lumellana le liliba tse fapaneng tsa solvelt le sampole. Ka lehlakoreng le leng, mashano a polasetiki a bobebe ebile a tloaetse ho anyesa, a etsa hore a sebelisoe tšimong kapa maemong ao khalase e sa lokelang.

Bohlokoa ba polokelo e nepahetseng

Taolo ea mocheso:Ho bohlokoa ho boloka litaolo tsa li-chromatography tsa chromatography sebakeng seo mocheso o bolokiloeng ka nako e telele. Liphetoho tsa tšohanyetso ka mocheso li ka baka tsitsipano ea mocheso, e ka lebisang karolong e meholo le liphetoho tsa mohlala. Ho fokotsa likotsi tsena, li-vaials li lokela ho bolokoa ka nepo kamoreng e laoloang ka mocheso kapa cabinet.

Tšireletso ea khanya:Ho pepesetsoa leseli la UV ho ka etsa hore batho ba lumelloe ka mehlala le ho ama maemo a ho tiisa manonyeletso. Ho sireletsa disampole ho tsoa leseling le tobileng le phofo, sebelisa amber kapa likhama tse mebala. Ntle le moo, li-vaes li lokela ho bolokoa mabokoseng a opaque kapa likhabinete ho fokotsa ho pepesetsoa leseli.

Taolo ea mongobo:Maemo a mongobo a phahameng a khothaletsa mongobo oa moipolli, e ka amang mohlala oa botsitso 'me e baka katoloso ea Septum. Boloka liemahale tikolohong e omeletseng 'me u nahane ka ho sebelisa likhakanyo lijana tsa polokelo ho boloka maemo a mongobo a le tlase.

Qoba Ho Siona:Li-vaels li lokela ho theoa hole le lik'hemik'hale tse ka bang kotsing, soe sollicalt kapa metsoako e makatsang. Sebelisa libaka tse inehetseng tsa polokelo bakeng sa mefuta e fapaneng ea disampole le ho kenya tšebetsong mangolo a nepahetseng ho tsamaisa boitseba le ho se sebetse hantle.

Boitšoaro bo nepahetseng: Ho boloka li-viesE lokile ho thibela ho tsoa kapa ho kopanya ka hare ho litaba le ho boloka botšepehi ba mohlala. Sena ke sa bohlokoa haholo ho li-vials le likokoana-hloko. Ho boloka li-veiare ka mokhoa o sa feleng kapa ka tlase ho ka sitisa ho tiisa le ho lebisa ho tahlehelo ea mohlala kapa tšilafalo.
Fumana mehato ea bohlokoa ho lokisa likhama tsa chromotography bakeng sa tlhahlobo e hlakileng ka mehato e tšeletseng e bonolo feela. Ntlafatsa kutloisiso ea hau le theknoloji ena ea bohlokoa bakeng sa litholoana tse tšepahalang!Mehato e 6 ho lokisa likhamati tsa chromiatography bakeng sa tlhahlobo

Ho sebetsana ka mokhoa o nepahetseng

Ho sebetsana le bonolo:Ha u sebetsana le litakatso tsa carmatography, qoba metsamao e mengata kapa ea tšohanyetso e ka bakang khatello ea maikutlo. Sebelisa li-parter tse bonolo tsa moea o bobebe kapa li-racks tsa patsi ho fokotsa kotsi ea ho oela kapa ho hlakotsa likhahla.
Qoba ho feta: Ha li-vielis tse tiisang, li sebelisa khatello e lekaneng le e bonolo ho netefatsa tiiso e sireletsehileng ntle le ho tiisa. Sekhahla se fetelletseng se ka senya septum kapa cap le ho sitisa bokhoni ba ho boloka tikoloho ea moea.

Lekola Bokhoni ba tiiso:Hlahloba likhafetsa bakeng sa matšoao a tšenyo ea tiiso e joalo ka mapetso, lichifi kapa li-septuress tse tsofetseng. Bakeng sa sampole tse mahlonoko le likhama tsa nako e telele tsa polokelo, etsa tlhahlobo e bonabisang 'me u nahane ka ho sebelisa liteko tsa tiiso joalo ka tlhahlobo ea khatello kapa ea ho qhalana.

Label le ho hlophisa: Li-vaebels hantleKa tlhaiso-leseling ea bohlokoa joalo ka lebitso la mohlala, letsatsi la pokello le litaelo tse itseng. Hlophisa lintho tse bonahalang e hlophisitsoe ka mokhoa o hlophisehileng maemong a polokelo (mocheso oa kamore, mocheso, oa lehloa, jj.

Sebelisa lisebelisoa tse lumellanang:Etsa bonnete ba hore li-cap, li-septures, 'me lisebelisoa tse ling tsa boima li lumellana le mekhoa ea sampole le mekhoa e meng ea ha e hlahlobisiwa. Sebelisa thepa e thibelang lik'hemik'hale bakeng sa lisebelisoa tsa ho thibela tšoaetso ea mohlala le ho sebelisana nakong ea polokelo le tlhahlobong.

Ho tseba hore na litšenyehelo tsa li-hipls li mapheoana a HPlc? Qoba ho sengoloa, litheko tse ka behang litheko tsa theko, le malebela a ho fumana likotlo tse theko e boima tsa HPLC e sebetsang:PLPC Valias Theko: Lipuisano tse 50 tse botsoang khafetsa

Mehopolo ea polokelo ea nako e telele

Ho purura ea koloi:Bakeng sa polokelo ea nako e telele ea li-samont tsa nako e telele, nahana ka ho itlhoekisetsang tasese ka khase e melelele e kang naetrojene kapa Argone. Ho ruruha ha khase ho itlhoekisa khase ho fokotsa oxidation, mongobo le ho nyenyefatsa sampole hape ho boloka botšepehi ba mohlala.

Boloka polokelo ea mahala:Haeba mehlala e lokela ho bolokoa ka mocheso o tlase, sebelisa likhama tse khethehileng tsa semela se hlophiselitsoeng ho mamella maemo a ho bata. Liaparo tsena hangata li na le marako a matla le litiiso ho thibela ho qhekella le ho itsamaisa ha ho senyeha ha serame se feteletseng.

TLHOKOMELISO EA BOTSOALLE:Etsa li-Protocol tsa ho qetela tsa ho qeta nako ea ho leka khafetsa, haholo-holo bakeng sa li-disam tse sebetsang kapa tse nang le mabetle. Ho etsa cheke tsa nako nako nako ea nako le nako ho tiisa hore likhamallo li lula li le thata ebile li thibela tahlehelo ea sampole kapa tšilafalo nakong ea polokelo.

Ho kenyelletsa mekhoa ena e qaqileng ho li-protocls tsa hau tsa laborator li tla leleka bophelo ba hauLithako tsa ChromatograftHopola botšepehi, 'me u eketse ho tšepahala ebile ho nepahetse ha liphetho tsa hau tsa sekaseka.

Lekola likarabo tse felletseng tsa lipotso tse 50 tse tloaelehileng mabapi le li-pilc tse phetoang sechabeng sena se rutang. Fumana lintlha tsa bohlokoa 'me u ntlafatse kutloisiso ea hau ka tšebeliso ea nalane ea HPLC le tlhokomelo:Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang
E fetileng:
Ho khetha filithara e nepahetseng ea syringe bakeng sa tlhahlobo ea lijo le ho feta
E 'ngoe:
Bokamoso ba France: Technology ea Trighches ho la Micron Syringter Filts
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