Tšimong ea chromatography e sebetsang ea chromatography (HPTC) le li-chromatograph tsa khase (gc) ke mekhoa e 'meli e hlahelletseng ea li-chankaneng. Mekhoa e meng ka bobeli e kenyelletsa karohano le tlhaiso ea metsoako, empa e sebetsa ka melao-motheo e fapaneng le ho hloka lisebelisoa tse ikhethang tse kang vaesita. Ho utloisisa liphapang lipakeng tsa li-hipc le GC ho bohlokoa ho netefatsa ts'ebetso le ho nepahala ha chromatographic.

Li-pilc tsa hipc

Sebopeho

Li-pilc tsa hipcli entsoe ka khalase ea boleng bo holimo, hangata e baka khalase kapa boitsebiso bo tšoanang, bo tsejoa ka ho hanyetsa ka lik'hemik'hale le ho hloka kelello. Lintho tsena li matsapa a mangata li etsa tlhahiso ea boitlhompho haholo hore li kopane le litekanyetso tsa bohloeki, nyenyefatsa kotsi ea tšoaetso ea mohlala kapa phetoho ea ho hlahlojoa. Ntle le moo, likotoana tsa polasetiki ea polasetiki tse entsoeng ka thepa e kang polypropylene li fumaneha bakeng sa lits'ebetso tse itseng, fana ka kotsi e kang ho fokotsoa ha lilallo le li-solvel.

Likaroloana

Moralo oa lipale tsa HPLC o totobatsa ho hlophiseha ka AutoSoslemplers e sebelisoang ka sistimi ea HPPc System. Ka tloaelo ba na le kotlo e tšesaane ea bonyane ba 8 ho isa ho tse ka bang 9 ho isa ho tse 9 'me li entsoe ka nepo ho lekana le koluoa ea HPPC Automoss kapa sampole slutter.

Li-vaiali tsa HPTC li na le li-cap tsa screw kapa li-cap tsa crimp ho fana ka maemo ho latela lintho tse ling tsa laboratori le litlhaloso tsa seletsa. Li-caps tse screw li loketse ho sebelisoa khafetsa ebile ho tsitsipana ha masapo a boloi ho tiisa tiiso e sireletsehileng, haholo-holo likopo tse matla.

Liphoofolo tsena li etselitsoe ho mamella likhatello tse phahameng tsa ho hlahlojoa ha Hplcs, ho boloka botšepehi ba mohlala le ho thibela ho nepahala ha data.

Kopo

Li-pilc tsa hipcSebeletse joalo ka lijana bakeng sa li-samp tsa mokelikeli ho kena ka sistiming ea HPPc. Sampole e kanna ea ba le lintho tse ngata, ho kenyelletsa meralo, likokoana-puo, lits'oants'o tsa tikoloho le metsoako ea lijo. Lisebelisoa tsa boleng bo holimo le kaho e nepahetseng ea li-valc tse hlakileng li netefatsa hore li-solvetina li na le li-solvet tse ngata le litlhahlobo tse fumanoeng ka li-chromatographs tse metsi.

Notlolla mahlomolo a 50 oa bohlokoa ka lebaka la tlhaiso-leseling ea Hplc ho sengoloa sena se felletseng. Imelang ka hare ho likarabo ka lisebelisoa, kamati le mekhoa e metle. Tataiso ea hau ea ho hlokomela li-valc tsa Hplc li qala mona!Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

Gc vaials

Tlhophiso

Gc vaialsHa e eoe haholo ho tsoa ho khalase ea borolicate, e etsoang ka khanyetso ea eona e ntle e kholo ea ho fifala le koronyana ea lik'hemi. Mofuta oa khalase ona o boloka botsitso ba mohlala le bohlokoa bakeng sa tlhahlobo ea metsoako e mengata e entsoeng ke khase ea khase ea khase. Khalase ea Borosilicate e ne e le haufi le mekhoa ea ho laola litekanyetso tsa boleng ba boleng ba ho netefatsa ts'ebetso e sa fetoheng mabapi le maemo a fapaneng a ts'ebetso.

Likaroloana

Moralo oa li-Gec tsa GC o qala ho phomola oa ho thibela likhohlano kapa tšilafalo ea 1112, ho nolofatsa sampole ho kenya liletsa tsa GC.

Li-vaialis tsa GC li tloaetse ho sebelisa mochini o koahetsoeng ka holimo, moo motho ea lumelloang ka mokhoa o sireletsehileng o sa sireletseha o tla sebelisa sesebelisoa se khethehileng sa koae. Mokhoa ona oa ho hloea o theha tiiso ea airlight e thibelang ho tsoa ka bongata ka ho hloka chelete e ngata haholo, e fokotsa molumo o mofubelu le ho lumella tekanyo e nepahetseng ea mohlala le ente.

Fumana tsebo ea ho khetha lintho tse ngata tse mpe bakeng sa litlhoko tsa hau! Bula batho ba bangata pakeng tsa likhahla tsa molaoli, liemahale tsa likhoele, le ho bokella li-scrange tse litšila tse bohlale. Etsa liqeto tse tummeng bakeng sa sephetho se nepahetseng. Mokokotlo hona joale!CRIMP Valia Vs. Viap Viss vs. cal cas e vael, ho khetha joang?

Kopo

Gc vaialsli etselitsoe ho tšoara mehlala e sa sebetseng kapa e sa fetoheng ea ho hlahlojoa ke chromatography ea khase. Sampole e ka ba le metsoako e fapaneng joalo ka hydrocarbons, meribeng e monate, e sitisa litlolo tse hlakileng, tse lumellang linkhoe tse nepahetseng le tse khothatsang.

Phapang ea Bohlokoa:

Ho netefatsa mechanism

HPLC Valias Sebelisa Likatse tsa Screw kapa tsa Trimp

Boholo ba aperture

Li-pilc tsa hipcE-ba le ho buoalla ho utetsa ho lekana nepo e ente ea li-apptions tsa li-spcosoplers tsa HPTc Aupole ho ntlafatsa mohlala oa ho kenyelletsa le ho fokotsa moea.

GC Valias e na le mecha e meholo ea ho nolofalletsa ho hlahisoa ea mehlala e sa sebetseng ho GC, e amohela lintlha tse kholo tsa bohlokoa le ho khothaletsa mouoane o sebetsang le karohano.

Mohlala

Li-vael tsa HPLC li loketse li-sampud tsa metsi li ile tsa kenngoa likhopolo tse phahameng tsa chromatography 'me li lumellana le mefuta e mengata ea li-solvent le litlhahlobo.

Gc vaialsli lumellana ka ho khetheha ka mokhoa o sa fetoheng kapa o sa tsebeng letho, ho boloka maemo a saense le litsebi tsa linomoro tsa tlhahlobo, 'me u fihlele tlhahlobo e phahameng ho senya lits'ebetso tsa Myriad.

Lekola lits'ebeliso tse 15. Ho tloha phamaneng ho ea ho tlhahlobo ea tikoloho, u fumane hore na likhama tsee li phetha karolo ea bohlokoa masimong a fapaneng. Mehlami ea marang-rang hona joale!Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

E fetileng:

Mabaka a 3 a ho sebelisa li-canromatograph tsa 4 tsa chromatography

E 'ngoe:

Cap e tlamang ke eng?