Mokhoa oa chrotography ke mokhoa oa bohlokoa o sebelisitsoeng masimong a saense ho tloha ho li-pharmaticas ho ea tlhahlobong ea tikoloho. Katleho ea chromatography e itšetlehileng haholo ka boleng ba likhahla le lisebelisoa tse ling tse sebelisitsoeng. Har'a likhetho tse fumanehang,4 ml chromatographsli ntse li tuma ka mabaka a 'maloa. Mona ke mabaka a mararo a matla ao u lokelang ho nahana ka ona a sebelisa liphaephe tsa li-chromagraphy tsa li-chromagraphy tsa hau bakeng sa liteko tsa hau le litlhahlobo

1. Mohlala o nepahetseng oa mohlala o lekaneng

Ho li-chromatography, ho sebetsa hantle ka mokhoa o nepahetseng oa mohlala ho bohlokoa ho fumana liphetho tse nepahetseng le tse khothatsang. Khetho ea mekhabiso e ama ka toro ea mehlala e ka kenngoeng tsamaisong ea li-chromatography. Boholo ba li-delis tse nyane tsa "sampole tse nyane, ha li-vies tse kholo li ka baka litšila. Li-variatography tsa 4 ml chromalete.

Molumo oa 4 oa ML o fana ka sebaka se lekaneng sa ho amohela molumo o tloaelehileng ntle le kotsi ea ho phatloha kapa ho felloa ke vaiss. Sena se tiisa hore Sampusi e kopantsoe hantle le ea solvent le ea libetsa ka katleho le chromatograph, ho hlakola karohano le ho lemoha.

Hore na u sebetse ka li-chromatography tsa chromatography (LC) kapa li-chromatograph tsa khase (gC), ho bohlokoa ho lokisa sampole e nepahetseng ho fihlela lichankana tse tšepahalang tsa chromatographic le ho qala. Mefuta e 4 ea ML e fana ka tlhahiso ea bophahamo, e lumella bafuputsi ho kenya mehlala ka sistimi ea li-chromatography. 2.

Fumana senotlolo sa tlhahlobo e nepahetseng le e tšepahalang le e tataisang botsofe bo loketseng ba likhamphani tse 6 Master theknoloji mme o phahamise liphetho tsa hau kajeno!Mehato e 6 ho lokisa likhamati tsa chromiatography bakeng sa tlhahlobo

2. Kamohelo le ho tsebahala

Ho se sebetse ha 4 ml celists tse 4 ml chromatigraphy ho ba etsa khetho e khethiloeng bakeng sa bafosi ba sebetsang ho sebelisa mekhoa e mengata ea li-chromatograph le lisebelisoa. Liphoofolo tsena li etselitsoe ho lumellana le mefuta e mengata ea li-auutisamplers le litsamaiso tsa ente tse sebelisoang khafetsa ho laeboeratograph tsa chromatograph.

Hore na o sebelisa chromatography e phahameng ea chromatography (hplc), mekhoa ea khase ea khase ea chromatographs Li ka imeloa habonolo ka boitokisetso ba ho hloka chelete ka boiketsetso kapa ho sebelisetsoa ka ente ea entetsi, ho fetoha le maemo le bonolo.

Ntle le moo, liphaemo tse 4 tsa chromatigraphy li fumaneha ka lisebelisoa tse fapaneng, ho kenyelletsa le khalase ea boroliral le li-polys tse kang koena e kang polypropylene. Phapang ena e etsa bafuputsi ba ho khetha e letellang ka ho fetisisa bakeng sa litlhoko tsa bona tse ikhethileng tsa liteko, botsitso ba sampole le ho hanyetsa mohlala oa sampole. 3.

3. Mmuso oa mohlala oa ho ntlafatsa letso le botšepehi

Ho boloka botšepehi ba mohlala ho bohlokoa bakeng sa liphetho tse nepahetseng le tse khothatsang li-chromatographicphic li-chromatographicphic li teng. Litoro tsa 4 tsa chromatography li etselitsoe likarolo tse fanang ka tšireletso ea mohlala khahlanong le mabaka a tikoloho a ka etsang hore ho be le mokhoa o botsitso le ho sekaseka ho hlaphoheloa.

Liphaere tsena li lula li na le methapo e sireletsehileng ea helmetic kapa ho koaloa le ho thibela mouoane, tšilafalo le ho sekaseka mokhoa oa ho boloka le ho hlahlojoa. Le ha mohlala o tsotella leseli, oksijene, kapa mongobo, mahobe a hemagement a ho lula a le mong ho tsoa ho li-clematographic.

Ntle le moo, moralo oa mofuta oa 4 ml ciamatography valia o fokotsa kotsi ea mekhoa ea mohlala le tšilafalo lipakeng tsa liente. Sena se etsa hore ho hlokahala botshepehi le ho ikatisa ha litholoana, lumella bafuputsi ba ka khotsofatsa ka boitshepo le boitsebahatso ba bona.

Ka kakaretso,4 ml chromatographsFana ka mokhoa o nepahetseng oa ho ntlafatsa mohlala o phethahetseng, o lumellana le mekhoa e fapaneng ea li-chromatography, 'me u itšireletse tšireletso ea mohlala. Ka ho khetha likhahla tse 4 tsa li-chrialis.

U batla likarabo mabapi le likhama tsa HPLC? Qobella ho sengoloa sa rona se felletseng se nang le leseli la 50 ho li-hilc vaialis le ho ntlafatsa sethala sa hau sa chromatography kajeno!Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Ke khetha mofuta o nepahetseng oa HPLC e nepahetseng joang?

E 'ngoe:

Phapang ke efe pakeng tsa li-valiat tsa HPLC le li-gc tse litšila?