Ukukhetha i-buffer efanelekileyo kwiSigaba se-HPLC yakho ye-HPLC kubalulekile ekufezekiseni ukwahlukana ngokupheleleyo kunye nohlalutyo lwakho iisampulu. I-Buffers inceda ukugcina i-PH ye-PH, ebaluleke kakhulu ekulawuleni imeko ye-ionis yohlalutyo lwakho, ngenxa yoko ichaphazela ixesha labo lokugcina kunye nobume be-piak. Apha ngezantsi kukho isishwankathelo esichaziweyo sendlela yokukhetha i-buffer efanelekileyo yesicelo sakho se-HPPC.

Ngaba uyazi malunga namalungiselelo esampulu ye-HPLC? Cofa eli nqaku ukuze ufunde ngakumbi: "Yonke into ofuna ukuyazi malunga namalungiselelo esampulu ye-HPLC"

Ukubaluleka kokukhetha i-buffer

I-buffer sisisombululo se-ph ye-hle ayitshintshi emva kongeza isixa esincinci se-acid okanye isiseko. Ngokusekwe kubume babo, ii-buffers zinokuhlelwa njenge-acidic okanye ezisisiseko. Ukusebenza kwe-buffer kugqitywa yi-pka yayo, okufanele ukuba ibe ngaphakathi kwiCandelo le-1 le-1 leSigaba seSoftware enqwenelekayo.

Izinto eziphambili ekufuneka ziqwalaselwe

I-1.PPH yo: I-PH yeSigaba seSoftware kufuneka ubuncinci ibe ziiyunithi ze-2 ze-ph kude kwi-PKA yohlalutyo. Oku kunceda ekucinezeleni i-icidic ye-acidic okanye ekuhlalutyeni okusisiseko, ukwandisa ukugcinwa kwabo kwikholamu. Umzekelo, ukuba uhlalutyo yicidi ebuthathaka nge-AGCE ye-4.5, usebenzisa isigaba se-2 kwi-PH malunga ne-2.0 singayigcina kwindawo engeyiyo i-UNIENT, ekwakheni ukugcinwa.

I-2.budforter uhlobo: Ii-buffers eziqhelekileyo ezisetyenzisiweyo kwi-HPPC zibandakanya:

I-phosphate buffers: isebenza kwi-PH Uluhlu lwe-2.0 ukuya kwi-8. Zilungele ngokukodwa izicelo ze-LC-UV.

I-Acetate Buffers: Ezi zinto zithandwa kakhulu ze-LC-UV ASESS kwaye zinokusetyenziswa kwi-prox efanayo ye-phosphate buffers.

I-buffers eziguquguqukayo: Izicelo ze-LC-ms, i-buffeler evuthayo, i-acetate, kunye ne-Ammonium Bicarbonate zikhethekile kuba zinokususwa ngokulula ngexesha lenkqubo ye-spectrometry.

I-3.Uvalbus yoxinzelelo: Uxinzelelo oluqhelekileyo lokuqala lwe-buffers kwi-HPPC phakathi kwe-25-50 mm. Ukugxininisa okuphezulu kunokuphucula ubume be-peak kunye nokusebenza kakuhle kodwa kungakhokelela ekuthini kuqine xa kuxutywe nezinyibilikisi. Kucetyiswa ukuba ugcine uxinzelelo lwe-buffer ngaphantsi kwe-25 mm ukuthintela iingxaki.

4.Unyulo lwe-Sulchemical Cety: Soloko usebenzisa izixhobo zeBanga eliPhezulu lweBanga eliPhezulu ukuthintela ungcoliseko kunye nokuqinisekisa iziphumo ezinokuthenjwa. Ukungcola kunokukhokelela kwizenzo ezingalindelekanga kunye nokwahluka kumaxesha okugcina.

Iziphumo zobushushu: Iqondo lobushushu linokuba nefuthe kwi-ph yezisombululo ze-buffer. Kubalulekile ukulungiselela kwaye ugcine i-buffers kubushushu obuqhelekileyo ukugcina impumelelo yabo.

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Iingcebiso ezisebenzayo ze-buffer

Indlela yokulungiselela: Ii-Buffers zinokulungiswa ngokuxuba izixa ze-acidic kunye neziseko ze-acidic kunye nezisiseko okanye ngokwezibalo isisombululo esisisiseko ne-acid. Kulumkele ukuhlengahlengisa i-PH emva kongeza izinyibilikisi eziphilayo, njengoko oku kunokukhokelela kwiziphumo ezingahambelaniyo.

Izisombululo ezitsha: Lungiselela izisombululo ze-buffer entsha rhoqo ukunciphisa ukukhula kwentsholongwane kunye nokucocwa kwamacandelo, ezinokuchaphazela ukusebenza kwe-chromatographic.

Ukuphepha ukulungela: Ii-Buffers ezithile, ezinje nge-titte, zinokulungisa izinto zentsimbi ezingaziziisitali zeenkqubo ze-HLLC. Ukuqinisekiswa kokuqhubela phambili kwenkqubo emva kokusebenzisa ii-buffrs ukuthintela ukonakala.

Ukuqukumbela

Ukukhetha i-buffer efanelekileyo kwiSigaba se-HPLC yakho ye-HPLC kubalulekile ekufezekisweni iziphumo ezinokuthenjwa kunye nokubuyiswa. Cinga ngohlobo lwe-buffer, uxinzelelo kunye nokucoceka xa ukhetha. Ngokulandela ezi ziKhokelo, unokuphucula indlela yakho ye-HLPC yokwahlukana ngempumelelo nokuhlalutya iisampulu zakho.

Ufuna ukwazi ixabiso le-HPLC Vials, nceda ujonge eli nqaku: Ixabiso le-vialc vials: I-50 kakhulu imibuzo ebuzwa rhoqo

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