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HPLC Syringe Filters: 0.45µm PTFE for Organic Solvents

Ensuring peak purity in liquid chromatography begins with effective sample preparation. The 0.45µm PTFE syringe filter is the industry standard for clarifying samples containing aggressive organic solvents. Designed for compatibility with chemically harsh matrices, these filters prevent column blockage and protect your HPLC instrumentation from particulate damage.

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Why Choose Hydrophobic PTFE Membranes?

 

Polytetrafluoroethylene (PTFE) is a material renowned for its exceptional chemical stability. Unlike nylon or cellulose acetate, a hydrophobic PTFE filter effectively resists degradation when exposed to strong acids, bases, and organic solvents like Acetonitrile or Methanol. This chemical inertness ensures that no extractables leach from the filter into your sample, guaranteeing the integrity of your HPLC analysis.

 

Key Specifications and Dimensions

 

To ensure fit with your manual syringes and analytical requirements, our filters are manufactured to precise tolerances.

Parameter Specification
Filter Media Hydrophobic PTFE (Polytetrafluoroethylene)
Pore Size 0.45 µm
Housing Material Medical Grade Polypropylene (PP)
Diameter 13mm or 25mm
Filtration Area 0.65 cm² (13mm) / 3.9 cm² (25mm)
Inlet/Outlet Female Luer Lock / Male Luer Slip

 

Application in HPLC Sample Prep

 

Using a syringe filter for HPLC is a critical step before injection. The 0.45µm pore size is universally accepted for removing fine particulates from the mobile phase and sample solution. This is particularly vital for standard HPLC systems (typically >3µm columns). For UHPLC applications involving sub-2µm columns, a finer 0.22µm filter may be required, but for general organic solvent filtration, the 0.45µm PTFE membrane offers the perfect balance of flow rate and retention efficiency.

 

Can I filter water with a hydrophobic PTFE syringe filter?

 

No, hydrophobic PTFE naturally repels water. It is designed for organic solvents. If you need to filter aqueous solutions, you must pre-wet the filter with alcohol or choose a Hydrophilic PTFE filter.

 

What is the difference between 13mm and 25mm syringe filters?

 

The difference is the sample volume capacity. Use a 13mm filter for small samples (up to 10mL) to minimize hold-up volume. Use a 25mm filter for larger volumes (10-100mL) to prevent back-pressure buildup.

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