How to Choose a Septa Pre-slit or Not?
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How to Choose a Septa Pre-slit or Not?

Jul. 31st, 2020
Aijiren offers Chromatography Septa in many different types and colors, using different materials, and the customer's main concern is whether I choose Pre-slit septa, this article will introduce the difference between Pre-slit septa and Non-slit septa, the article is for reference only, the specific choice of that type of Septa also need to be self-judgment.

What is Pre-Slit Septa

Pre-slit septa
is ideal for automatic sampleing because Septa is kept sealed between Vials and Caps, and pre-slit processing makes it easy to puncture the sample feeder's injection into HPLC Vials for sampling. Despite the slit, Septa is also able to maintain the sample seal in HPLC Vial without creating a vacuum in the air pressure inside the HPLC Vial when sampling the auto-injection needle.

Advantages of pre-slit septa

One of the main advantages of
pre-slit septa is that they can reduce the risk of coring, which is the formation of a small piece of septa material that can dislodge and contaminate the sample. When the septa are punctured by the needle repeatedly in the same spot, the septa material can distort and create a tiny core. Pre-slit septa reduce the chance of coring because the needle enters the septa through the pre-cut slit.


Which Tpye we can Offer for Pre-slit Speta

Aijiren offers two types of
pre-slit Septa, single slit and cross slit, such a slit can guide the injection needle accurately punctured Septa into the HPLC Vials, without a thin automatic injection broken, or the cushion falls off into the HPLC Vial contaminated sample.

Non-slit septas

Non-slit septas
help reduce carryovers from vials to vials because the re-sealing properties of the septum act as a skige for wiping the solution from the outside of the needle.

Typically, a non-slit septum exhibits resealing capabilities for a limited number of needle punctures , but it is recommended to use a new non-puncture septum when storing for long periods of time in vials.

How to Choose Pre-slit septa and Non-slit septas

There are a number of things to take into account when deciding between pre-slit and non-pre-slit septa, including the sample type, needle gauge, and injection frequency.

Sample Type: Pre-slit septa may be necessary for some samples to lessen the chance of coring, but not necessarily for all samples. 

Needle Gauge: A bigger needle gauge can need more force to get through non-pre-slit septa, which could cause damage to the septa or sample contamination. A pre-slit septum might be the best choice in this situation. 

Injection Frequency: A pre-slit septa may be more appropriate for applications requiring repeated injections since it can lessen the effort needed to penetrate the septa with a needle and increase the septa's lifespan.

If you wish to acquire extensive knowledge about PTFE/Silicone septa, I suggest consulting this article:Premium PTFE and Silicone Septa: Reliable Sealing Solutions

5 Frequently Asked Questions (FAQs) of Pre-slit Septa

1. Are Pre-Slit Septa Reusable? 

Primary Pre-slit septa are generally intended for one use only and any attempt to reuse one could compromise the integrity of its seal, increasing contamination risks and decreasing accuracy and reliability results. Therefore it is advisable to use new septa for each sampling or analysis process for accurate and reliable results.

2.Are pre-slit septa autoclavable?

The autoclavability of pre-slit septa depends on their material composition; some septa may withstand autoclaving temperatures while others may degrade over time and lose sealing properties, so it is wise to consult your septa manufacturer's specifications for autoclavability before using any autoclaved product. If autoclaving is required, alternative septa materials or sterilization methods may also be available.

3. Can pre-slit septa be used with volatile or reactive samples? 

Pre-slit septa come in various materials that are designed specifically to be compatible with volatile or reactive samples, making it important to choose one suitable for such samples based on properties like high volatility or chemical reactivity. It's best to seek manufacturer recommendations or consult a supplier when making this selection; otherwise, it could prove disastrous!

4. How should I store pre-slit septa? 

Pre-slit septa should be stored in an ideal environment: clean, dry, and cool temperatures to preserve their integrity and preserve sealing properties. Exposure to high heat or humidity levels or direct sunlight could degrade them over time; for added protection, it would be prudent to store them in their original packaging or airtight containers to safeguard them from contamination while maintaining sealing properties.

5. Can pre-slit septa be used with different needle sizes?

Yes, pre-slit septa are designed to accommodate various needle sizes. It is important that when selecting pre-slit septa for use with particular needle sizes the appropriate slit size matches up. This ensures a secure seal against leakage or damage of the septa. To select the right septa for your needle diameter use, consult either the manufacturer's specifications or consult a supplier in order to select an ideal septa solution.

What to Pay a Attention To:

1. It is important to note that Silicone rubber can easily absorb chemicals from the environment.
2. Be careful when storing caps before use.
3. Do not store septums where chemical vapors are present for long periods of time.
4. I hope this article will be helpful for you to buy Septa.


The precise requirements of your application will determine whether you choose
pre-slit septa or non-slit septa. While non-slit septa are advised for applications requiring the highest level of sample integrity, pre-slit septa are more practical for normal use. You may make an informed choice and guarantee the accuracy and dependability of your chromatography analysis by being aware of the benefits and drawbacks of each type of septa.

Contact us now

If you want to buy 
Pre-slit septa of Aijiren, please contact us by the following five ways. We will reply to you as soon as possible.

1.Leave a message at our official website
2.Contact our online customer service at lower right window
3.Whatsapp me directly:
4.Mail me
5.Call me:8618057059123

To gain a comprehensive understanding of PTFE/Silicone septa, I recommend referring to the following article: Premium PTFE and Silicone Septa: Reliable Sealing Solutions