Jul. 10th, 2019
Chromatographic analysis is a separation and analysis technique for multi-component mixtures. It mainly uses the difference in boiling point, polarity and adsorption coefficient of each component in the sample, so that the components are separated in the column, and the separated components are detected by the detector, so that The component mixture is subjected to qualitative and quantitative analysis.
Because of its high separation efficiency, fast analysis speed and low sample consumption, the analytical method has been widely used in petrochemical, biochemical, medical and health, sanitary quarantine, food inspection, environmental protection, food industry, medical clinical, etc. department. Gas chromatography solves problems such as quality inspection, scientific research, pollution detection, and production control of industrially produced intermediates and industrial products in these fields.
1) Basic knowledge about vials:
The requirements for the vial are accurate, can withstand a certain pressure, good sealing performance, and no adsorption to the sample. The appropriate one should be selected according to the requirements of the instrument and the condition of the sample. Sealing cap: In order to prevent the gasket from adsorbing the sample components, the gasket with PTFE or aluminum inner liner is used. The selection conditions (temperature) and the specific conditions of the sample depend on the sample. Routine analysis can be done with a butyl rubber mat and a line of silicone rubber mats for trace analysis. If necessary, a blank analysis is performed to confirm that the volatiles in the gasket do not interfere with the analysis.
2) About how to choose a sample vial
First, we need to understand that the vials are mainly jaw cap vials, threaded cap vials and bayonet caps.
If you want a general-purpose and acid-resistant type, you can choose a glass sample bottle;
If used for very small injection volumes, a small inner liner is required;
Silaneization/deactivation is required if it is used for samples and trace analysis that are easily bonded to the wall of a glass bottle;
If used for light sensitive samples, a brown bottle is required;
If it is used for alcohol samples or water soluble solvents, polypropylene is required.
3) From how to clean the sample bottle
In fact, when we do liquid chromatography, the hplc vials
is a one-off, but the cost is too high, so now we need to use a repeated sample vial, we have to better clean. We can try to use organic reagents for cleaning. Ethanol can be used for cleaning. If it is used urgently, it can be washed with ethanol and then blown dry with a hair dryer.