To make this important question clear, two of experts in the United States and the UK conducted a research study and presented the results of Using Re-washed Vials and Closures in a technical note 20670.
Comparing the use of washed chromatography vials and closures with new vials using gas chromatography and HPLC instruments,They describe problems with numerous images and tables,also did full mass range scans of the vials.Really interesting, The photos of re-used vials showing glue remnants from labels, screw thread damage, and clouding of the glass . But, the really fascinating were of the 100x magnifications showing the abraded surfaces of clear and amber glass vials before use and after cleaning of a used vial.
Some conclusions about chromatography vials and closures include the following findings:
· Additional components were found in the GC-MS blanks in all the test cases.
· Downtime for the GC instrument was increased due to an increased rate of injection liner replacement.
· Even small volume injections showed the introduction of additional peaks in the UV mode for the HPLC instrument testing.
· Contamination of the injection from the vials was measurably increased when re-using septa plus increased evaporative losses.
In a word,Experts recommendation: “Cost of Time spent in retests and chemical analysis failure that far greater than any perceived benefits of re-using chromatography vials and closures.”