What is a good HPLC vial?
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What is a good HPLC vial?

Apr. 19th, 2023

I. Introduction 


HPLC, or high-performance liquid chromatography, is a technique widely used in analytical chemistry to separate and identify components of a test sample. HPLC results depend on the equipment used, including the vials which hold the samples. For accurate and reliable HPLC results, it is crucial to choose the right HPLC vial


This article will highlight the most important considerations when selecting HPLC vials. We will look at the different types of HPLC Vials and their main features. We will also provide recommendations on how to choose and use HPLC Vials for optimal performance and accurate outcomes.


 II. Types of HPLC Vials


Glass vials are the most commonly used type of HPLC vials, as they offer excellent chemical resistance, low extractable/leachable levels, and high clarity, which makes them ideal for many applications. The most common material used for HPLC vials is borosilicate, due to its resistance to thermal and chemical attack. Amber glass vials are ideal for sensitive samples and offer great protection from light. However, they are less transparent than clear glass vials.


Plastic vials also have a high demand due to their light weight and affordability. Polypropylene vials are commonly used. They can be compatible with many aqueous or organic solvents but not all. They are made of polyethylene (PE), which is ideal for storage and transporting samples, but not for HPLC due to its low chemical resistance.


The material used for HPLC vial is determined by the type of sample, the detection system, and the chromatography method. To avoid contamination, it's crucial to ensure that the vial material is compatible with the solvents or reagents being used.


III. What is a good HPLC vial??


A good HPLC vial should be compatible with the solvents used in the chromatography process, have low extractable/leachable levels, and consistent manufacturing quality. To prevent sample loss during analysis, the vials must fit properly with the chromatography equipment and accessories like caps and septa.

1.Compatibility of Solvents


HPLC vials must be compatible with the solvents being used for the chromatography. Solvents can degrade vial materials or cause contaminants to leach out, which could affect the results and sample. Glass vials tend to be more compatible with solvents. Some plastic vials that are compatible with solvents of a wider range are available.

2.Low Extractables/Leachables


Leachables can release compounds from the vial into the sample while extractables can release compounds into the solvent. Both can lead to false positive or false negative results, making it essential to choose vials with low extractables/leachables. Glass vials are known to have lower levels of extractables/leachables than plastic vials.

3.Consistent manufacturing quality


The HPLC vials' manufacturing process can have an impact on their performance as well as the reliability of the analytical results. You should only buy vials made by manufacturers who have a good reputation for quality control. It is important to choose vials from manufacturers who have a reputation for quality control and adherence to industry standards.

4.The Chromatography System must be properly fitted to the camera.


HPLC vial is available in a variety of designs including screw top, snap top and shell vials. Each has its own unique characteristics. When choosing vials, it is important to ensure that they fit with the chromatography system as well as accessories like caps and septa. The vials must fit correctly to ensure that they seal properly and that no sample is lost during analysis.

Delve into the world of 2ml 9mm HPLC vials with this informative article. Explore their features and applications in analytical chemistry:1.5mL 9mm Short Thread Vial ND9

IV. The proper handling and storage of HPLC Vials


To ensure accurate and reliable results, it is important to handle and store HPLC vials correctly. Here are a few best practices you should consider:


1. Avoid light exposure: Light can degrade some samples. Store vials in dark places or cover them using a dark material.

2. Vials should be stored in the correct temperature range. Extreme temperatures may also affect the quality of the samples.  

3. Avoid moisture. Moisture is a leading cause of contamination. It is important to store vials in an environment that's dry.

4. Avoid overtightening the cap. The caps can break if they are over-tightened. Caps must only be tightened to the point of creating a seal.  

5. Label vials correctly: To avoid confusion and to ensure traceability, it is important to label vials in the correct way. Label vials clearly with the sample name, date, and any other relevant information. 

6. Before using vials, it is important to inspect them for damage or defects. 


It is important to use certified vials to ensure they are compatible with the chromatography system and meet specifications. Proper handling and storage of HPLC vials can help to prevent contamination and produce accurate and reliable results.


V. Best Practices for HPLC Vial Use 


It is essential to use HPLC vials so that they perform optimally and provide accurate results. HPLC vials should be kept away form heat and sunlight. Moisture and light exposure can impact the accuracy and quality results. 


Use only certified vials. Certified vials have been tested for performance and quality. These vials can be used for analytical methods requiring accuracy and precision.


Label vials properly: It is important to label vials correctly with pertinent information, such as the sample name, concentration and date of preparation. This will help to avoid confusion or errors when analyzing.


Check vials before use for any damage or defects. Any vials with signs of damage or defect should be discarded.


Following these best practices will help users ensure that HPLC vial can be used in a way that maximizes accuracy and performance in chromatography analyses.


VI. How to choose the right HPLC vials


It is important to take into account the type of sample and the compatibility with solvent when choosing HPLC vials. If the sample is acidic, it is better to use vials of borosilicate, which is more resistant to chemical attacks than other types of glass. If the sample is volatile then vials made from polypropylene or other materials with low adsorption capacities may be better suited.


Budget is another factor to be considered. Although some vials are more expensive than other, the total cost of chromatography is not affected by the price of the vial. Incompatible or low-quality vials may lead to inaccurate and expensive results.


HPLC vials are available from several well-known brands, each of which has its own features and benefits. Waters, Agilent Thermo Fisher and Shimadzu are some of the most popular brands. Before making a purchase, it is important to compare and research the different brands.


VII. Conclusion


Selecting the right HPLC vial for your chromatography system is critical to obtaining accurate and reliable results. When selecting HPLC vials, key factors include the material and design of the vial, compatibility with chromatography systems and sample types, as well as quality control measures. Analysts can achieve optimal results and performance by following the best practices when handling and storing HPLC Vials, including avoiding light, moisture and extreme temperatures and labeling and inspecting vials prior to use.


There are many HPLC vial options and brands available. It is important to compare and research the different brands in order to choose the right option for your analytical needs. Analysts can achieve reliable and consistent results by selecting compatible, high-quality vials, and following proper storage and handling techniques.

Unlock The Answers To The 50 Most Frequently Asked Questions About HPLC Vials In This Comprehensive And Informative Article:50 Most Frequently Asked Questions on HPLC Vials

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