Are you using HPLC vials in your laboratory?
Do you have some questions about HPLC vials but don't know where to start looking for answers?
In this article, we have compiled 50 of the most frequently asked questions about HPLC vials to help you navigate the world of chromatography.

Basics of HPLC Vials

What are HPLC vials?

HPLC vials are small containers used to hold liquid samples for high-performance liquid chromatography (HPLC) analysis.

What is the purpose of HPLC vials?

HPLC vials are used to contain liquid samples for HPLC analysis, ensuring accurate results.

What are HPLC vials made of?

HPLC vials are made of different materials including glass, polypropylene, and polyethylene.

What are the different types of HPLC vials?

The different types of HPLC vials include clear, amber, crimp top, screw top, flat bottom, round bottom, conical, and straight.

What is the difference between clear and amber HPLC vials?

Clear HPLC vials allow for easy visual inspection of the sample while amber HPLC vials protect light-sensitive samples from degradation.

What is the typical size of an HPLC vial?

HPLC vials come in different sizes ranging from 1.5 mL to 4 mL.

What is the maximum pressure that an HPLC vial can withstand?

HPLC vials can withstand pressures up to 6000 psi.

What is the maximum temperature that an HPLC vial can withstand?

HPLC vials can withstand temperatures up to 121°C.

What is the shelf life of HPLC vials?

HPLC vials have a shelf life of 3-5 years.

How should I store HPLC vials?

HPLC vials should be stored in a cool, dry place away from direct sunlight.

Choosing HPLC Vials

What factors should I consider when choosing HPLC vials?

When choosing HPLC vials, consider the sample volume, compatibility with the solvent, and whether the sample is light-sensitive.

How do I know which type of HPLC vial to use for my application?

Choose the type of HPLC vial based on the sample volume, compatibility with the solvent, and whether the sample is light-sensitive.

What is the difference between screw caps and crimp cap HPLC vials?

Crimped HPLC vials require a crimping tool to seal the vial while screw-cap HPLC vials can be sealed manually.

What is the difference between flat and rounded bottom HPLC vials?

Flat-bottom HPLC vials are more stable while round-bottom HPLC vials are easier to handle.

What is the difference between conical and straight HPLC vials?

Conical HPLC vials have a tapered shape while straight HPLC vials have a straight shape.

What is the difference between deactivated and non-deactivated HPLC vials?

Deactivated HPLC vials have a special coating to reduce sample adsorption while non-deactivated HPLC vials do not.

What is the difference between pre-slit and non-pre-slit septa?

Pre-slit septa have a slit in the center for easier needle penetration while non-pre-slit septa require the needle to puncture the septa.

What is the role of septa in HPLC vials?

Septa provides a barrier between the sample and the environment, preventing contamination and evaporation.

Are there any special considerations for choosing HPLC vials for volatile samples?

Choose HPLC vials with a low-bleed septum for volatile samples.

Are there any special considerations for choosing HPLC vials for biopharmaceutical applications?

Choose HPLC vials with a low-adsorption surface for biopharmaceutical applications.
 

Using HPLC Vials

How do I prepare an HPLC vial for use?

Clean the vial with an appropriate solvent, insert the septum, and fill the vial with the sample.

How do I choose the right needle for my HPLC vial?

Choose a needle with an appropriate gauge and length for the sample volume and viscosity.

How do I choose the right syringe for my HPLC vial?

Choose a syringe with an appropriate volume and compatibility with the solvent.

How do I insert a septum into an HPLC vial?

Use a septum inserter tool to insert the septum into the HPLC vial.

How do I remove a septum from an HPLC vial?

Use a septum removal tool to remove the septum from the HPLC vial.

How do I clean an HPLC vial?

Clean the HPLC vial by rinsing it with an appropriate solvent and allowing it to dry completely.

Can HPLC vials be reused?

It is not recommended to reuse HPLC vials as they can lead to inaccurate results due to contamination.

How many times can I reuse an HPLC vial?

Use a new HPLC vial for each analysis to avoid contamination and ensure accurate results.

How do I dispose of used HPLC vials?

Dispose of used HPLC vials according to local regulations for hazardous waste.

How do I prevent contamination of my HPLC vials?

Minimize exposure of the HPLC vial to the environment and handle the vial with clean hands.

Troubleshooting HPLC Vials

Why do my HPLC vials leak?

Ensure the septum is properly inserted and the vial is not overfilled.

How do I prevent my HPLC vials from breaking?

Handle the HPLC vial with care and avoid dropping or bumping it.

How do I prevent my HPLC vials from cracking?

Store the HPLC vials in an appropriate container and avoid exposure to extreme temperatures.

How do I prevent my HPLC vials from sticking together?

Store the HPLC vials in a dry place away from moisture.

Why do my HPLC vials get stuck in the autosampler?

Ensure the HPLC vial is compatible with the autosampler and the vial is properly seated.

How do I remove a stuck HPLC vial from the autosampler?

Use a vial removal tool to remove the HPLC vial from the autosampler.

How do I prevent my HPLC vials from getting cross-contaminated?

Use a new HPLC vial for each sample and avoid touching the septum.

How do I prevent my HPLC vials from getting damaged during transport?

Use an appropriate container for transport and handle with care.

How do I prevent my HPLC vials from losing their seal?

Ensure the septum is properly inserted and the vial is not overfilled.

Why do my HPLC vials get blocked?

Blocked HPLC vials can be caused by sample precipitation or contamination.

Quality Control of HPLC Vials

How do I perform quality control on HPLC vials?

Perform quality control tests such as leak tests and pressure tests.

What are the common quality control tests for HPLC vials?

Common quality control tests include leak tests, pressure tests, and compatibility tests.

How do I know if my HPLC vials are of good quality?

Choose HPLC vials from a reputable supplier and perform quality control tests.

How can I verify the compatibility of my HPLC vials with my instrument?

Consult the instrument manual or contact the instrument manufacturer to determine the compatibility of HPLC vials with the instrument.

How can I ensure the consistency of my HPLC vials from batch to batch?

Choose HPLC vials from a reputable supplier and perform quality control tests on each batch.
 

Purchasing HPLC Vials

Where can I buy HPLC vials?

HPLC vials can be purchased from laboratory supply companies or online suppliers.

What factors should I consider when purchasing HPLC vials?

Consider the price, quality, and compatibility with your application when purchasing HPLC vials.

How do I compare prices of HPLC vials from different suppliers?

Compare the price per unit and shipping costs from different suppliers.

How do I evaluate the quality of HPLC vials from different suppliers?

Choose HPLC vials from a reputable supplier and perform quality control tests.

How do I choose a reputable supplier of HPLC vials?

Choose a supplier with a good reputation, positive reviews, and a history of providing quality products.

 

In conclusion, HPLC vials are an essential component of any chromatography laboratory. By understanding the basics of HPLC vials, choosing the right type of vial, using them correctly, troubleshooting common problems, performing quality control tests, and purchasing from reputable suppliers, you can ensure accurate and reliable results in your HPLC analysis.
 

Contact us now

If you want to buy HPLC vials of Aijiren, please contact us by the following five ways. We will reply to you as soon as possible.

1.Leave a message at our official website
2.Contact our online customer service
3.Whatsapp me directly: +8618057059123
4.Mail me directly:market@aijirenvial.com
5.Call me:8618057059123